Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis

doi:10.1186/gb-2004-6-1-r6

Genome Biology
Volume 6
Issue 1

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Caldwell RB
Kierzek AM
Arakawa H
Bezzubov Y
Zaim J
Fiedler P
Kutter S
Blagodatski A
Kostovska D
Koter M
Plachy J
Carninci P
Hayashizaki Y
Buerstedde JM

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Caldwell RB
Kierzek AM
Arakawa H
Bezzubov Y
Zaim J
Fiedler P
Kutter S
Blagodatski A
Kostovska D
Koter M
Plachy J
Carninci P
Hayashizaki Y
Buerstedde JM
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Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis

Randolph B Caldwell^*¹ , Andrzej M Kierzek^*²^,3 , Hiroshi Arakawa¹ , Yuri Bezzubov¹ , Jolanta Zaim² , Petra Fiedler¹ , Stefan Kutter¹ , Artem Blagodatski¹ , Diyana Kostovska¹ , Marek Koter¹ , Jiri Plachy⁴ , Piero Carninci⁵^,6 , Yoshihide Hayashizaki⁵^,6 and Jean-Marie Buerstedde¹

¹Institute of Molecular Radiobiology, GSF, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany

²Laboratory of Systems Biology Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warszawa, Poland

³School of Biomedical and Molecular Sciences, University of Surrey, Guildford GU2 7XH, UK

⁴Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, 16637 Praha, Czech Republic

⁵Laboratory for Genome Exploration Research Project, Genomic Sciences Center and Genome Science Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan

⁶Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

author email corresponding author email* Contributed equally

Genome Biology 2004, 6:R6doi:10.1186/gb-2004-6-1-r6


Published:	23 December 2004

Subject areas: Genome studies, Model organisms, Immunology, Genetics

Abstract

A large number of cDNA inserts were sequenced from a high-quality library of chicken bursal lymphocyte cDNAs. Comparisons to public gene databases indicate that the cDNA collection represents more than 2,000 new, full-length transcripts. This resource defines the structure and the coding potential of a large fraction of B-cell specific and housekeeping genes whose function can be analyzed by disruption in the chicken DT40 B-cell line.