Open Access Method

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis

Randolph B Caldwell1, Andrzej M Kierzek23, Hiroshi Arakawa1, Yuri Bezzubov1, Jolanta Zaim2, Petra Fiedler1, Stefan Kutter1, Artem Blagodatski1, Diyana Kostovska1, Marek Koter1, Jiri Plachy4, Piero Carninci56, Yoshihide Hayashizaki56 and Jean-Marie Buerstedde1*

  • * Corresponding author: Jean-Marie Buerstedde buerstedde@gsf.de

  • † Equal contributors

Author Affiliations

1 Institute of Molecular Radiobiology, GSF, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany

2 Laboratory of Systems Biology Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warszawa, Poland

3 School of Biomedical and Molecular Sciences, University of Surrey, Guildford GU2 7XH, UK

4 Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, 16637 Praha, Czech Republic

5 Laboratory for Genome Exploration Research Project, Genomic Sciences Center and Genome Science Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan

6 Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

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Genome Biology 2004, 6:R6  doi:10.1186/gb-2004-6-1-r6

Published: 23 December 2004

Abstract

A large number of cDNA inserts were sequenced from a high-quality library of chicken bursal lymphocyte cDNAs. Comparisons to public gene databases indicate that the cDNA collection represents more than 2,000 new, full-length transcripts. This resource defines the structure and the coding potential of a large fraction of B-cell specific and housekeeping genes whose function can be analyzed by disruption in the chicken DT40 B-cell line.