|
Comparison of different probability thresholds that were applied to the MS results |
||||
| Probability |
≥ 0.70 |
≥ 0.90 |
≥ 0.95 |
≥ 0.99 |
| Total number of passing spectra |
245724 |
224793 |
211674 |
179410 |
| Peptides |
31290 |
26840 |
25022 |
21598 |
| Distinct peptides with protein sequence matches |
29393 |
25754 |
24172 |
21030 |
| Number of mapped proteins |
11612 |
9747 |
9016 |
8134 |
| Number of simple reduced proteins |
7097 |
5826 |
5383 |
4845 |
| False-positive estimate MS/MS spectra |
2.4% |
0.9% |
0.05% |
0.01% |
| False-positive estimate with protein sequence matches |
<16% |
<6% |
<3% |
<0.8% |
Desiere et al. Genome Biology 2004 6:R9 doi:10.1186/gb-2004-6-1-r9 |
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