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Open Access Research

The two tempos of nuclear pore complex evolution: highly adapting proteins in an ancient frozen structure

Eric Bapteste1, Robert L Charlebois12, Dave MacLeod1 and Céline Brochier3*

Author Affiliations

1 Canadian Institute for Advanced Research Program in Evolutionary Biology, Department of Biochemistry and Molecular Biology, Dalhousie University, College Street, Halifax, Nova Scotia, B3H 1X5 Canada

2 Genome Atlantic, Department of Biochemistry and Molecular Biology, Dalhousie University, 5850 College Street, Halifax, Nova Scotia, B3H 1X5, Canada

3 EA EGEE (Evolution, Génome, Environnement), Centre Saint-Charles, Université Aix-Marseille I, place Victor Hugo, 13331 Marseille Cedex 3, France

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Genome Biology 2005, 6:R85  doi:10.1186/gb-2005-6-10-r85

Published: 30 September 2005

Abstract

Background

The origin of the nuclear compartment has been extensively debated, leading to several alternative views on the evolution of the eukaryotic nucleus. Until recently, too little phylogenetic information was available to address this issue by using multiple characters for many lineages.

Results

We analyzed 65 proteins integral to or associated with the nuclear pore complex (NPC), including all the identified nucleoporins, the components of their anchoring system and some of their main partners. We used reconstruction of ancestral sequences of these proteins to expand the detection of homologs, and showed that the majority of them, present all over the nuclear pore structure, share homologs in all extant eukaryotic lineages. The anchoring system, by contrast, is analogous between the different eukaryotic lineages and is thus a relatively recent innovation. We also showed the existence of high heterogeneity of evolutionary rates between these proteins, as well as between and within lineages. We show that the ubiquitous genes of the nuclear pore structure are not strongly conserved at the sequence level, and that only their domains are relatively well preserved.

Conclusion

We propose that an NPC very similar to the extant one was already present in at least the last common ancestor of all extant eukaryotes and it would not have undergone major changes since its early origin. Importantly, we observe that sequences and structures obey two very different tempos of evolution. We suggest that, despite strong constraints that froze the structural evolution of the nuclear pore, the NPC is still highly adaptive, modern, and flexible at the sequence level.