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Large-scale approaches for glycobiology

Christopher T Campbell and Kevin J Yarema*

Genome Biology 2005, 6:236 doi:10.1186/gb-2005-6-11-236

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Research article   Open Access

Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection

Gudrun Schiedner, Sabine Hertel, Corinna Bialek, Helmut Kewes, Gero Waschütza, Christoph Volpers BMC Biotechnology 2008, 8:13 (12 February 2008)

Abstract | Full text | PDF | PubMed | Editor’s summary

Stable cell lines can be generated independent of antibiotic selection by immortalizing human amniocytes, collected during amniocentesis, via the introduction of adenoviral E1 functions, offering a promising new method for use in the pharmaceutical industry.

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