Table 4

Primers used for bisulfite sequencing

SIN3-11193

(A011193)

G->A: 5'-CCTCCTTCGTTGACCTGTCTTCATCGCAATGACTCAGCATAG-3'

C->T: 5'- GTCTTCTAATCAAGTTTAGTTATGTTAATGTTTTTGGATAGAAC-3'

SIN3-5300

(A005300)

G->A: 5'-TTCATTTGTTACCTACTATCATTTTCAAGAACGAAACAATG-3'

C->T: 5'-TAGTAGTTGTTCTCATCTTGTTTTTGGCAACTGGACGTGTC-3'

229R1

(A000229)

G->A:5'- CACCATGTTCTAGCCCTTGTTCGGTCGTCGTTCCTTCCGTGG-3'

C->T: 5'- AAAAGAAAGGCGTCGTGGAATCACCACTAGCTACAACCGC-3'

229R2

(A000229)

G->A:5'- TTAGAGCTTGTTTTCATTACCTTCTTCACACAACCTCCAAG-3'

C->T: 5'- TTTCAGGGTATCATGGTTCTCGACAAAGTAGGGTTATTATC-3'

TA11-4217

(A004217)

G->A:5'- CAACATAAGATTGTAGCCTTCCATCCTTGACCACGCTTTG-3'

C->T: 5'- TCTTAAGATAGGAGATGATGTGTAGGAATGGTTTCTGGCAC-3'

MU-4802

(A004802)

G->A:5'- AGCCATTATCATGTCCATCTGATCCTTCTACATGCCCTTG-3'

C->T: 5'- TATGTGAACGACTCATACACAAGAAATAGGTGGCGAGAAAC-3'

MU-PCR

(57802433)

G->A:5'- CACCAGCTCGAACACCACCAACAGATTCCTTGTAAATCTG-3'

C->T: 5'- GATGGAGCGAGTGACGGGGATGAAGAGTCTAGTGTGTGCAC-3'

To amplify bisulfite-treated DNA, primers were synthesized with G→A (first sequence of the pair or C→T (second sequence), except for CGs and CNGs, which were synthesized with G→R or C→Y, respectively.

Tran et al. Genome Biology 2005 6:R90   doi:10.1186/gb-2005-6-11-r90