Figure 5.

MESA overexpression in CD36 parasites. (a) Western blot of non-synchronized parasites from FCR3-CD36 and FCR3-CSA parasites. PfHsp70 protein is included as a loading control. A monoclonal antibody (Pf12.8B7.4) against MESA [60] detects approximately 2-4 times more protein in CD36 compared with CSA panned parasites. (b) Immunofluorescence for MESA protein in FCR3-CD36 and FCR3-CSA parasites. The 488-labeled secondary shows that MESA is considerably more abundant in CD36-compared with CSA-panned parasites. The intracellular distribution of MESA is the same in both parasite populations - with most labeling localizing to the periphery of infected erythrocytes.