Figure 3.

Transcription of Sly is reduced or absent in the MSYq deficient males. (a) Northern blot of total testis RNA probed with the microarrayed Sly cDNA clone MTnH-K10 and with an actin probe as a loading control. Hybridization to the Sly probe is clearly reduced in 2/3MSYq^- males and is further markedly reduced in the two models with more severe MSYq deficiency. (b) Reverse transcriptase polymerase chain reaction analysis of testis cDNA with primers that distinguish between Sly and Xmr transcripts and with Hprt primers as an amplification control. Some Sly transcripts are retained in 9/10MSYq^- males but they are absent in MSYq^- males.