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The design of transcription-factor binding sites is affected by combinatorial regulation

Yonatan Bilu1 and Naama Barkai12*

Author Affiliations

1 Department of Molecular Genetics, Weizmann Institute of Science, 76100 Rehovot, Israel

2 Department of Physics of Complex Systems, Weizmann Institute of Science, 76100 Rehovot, Israel

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Genome Biology 2005, 6:R103  doi:10.1186/gb-2005-6-12-r103

Published: 2 December 2005

Abstract

Background

Transcription factors regulate gene expression by binding to specific cis-regulatory elements in gene promoters. Although DNA sequences that serve as transcription-factor binding sites have been characterized and associated with the regulation of numerous genes, the principles that govern the design and evolution of such sites are poorly understood.

Results

Using the comprehensive mapping of binding-site locations available in Saccharomyces cerevisiae, we examined possible factors that may have an impact on binding-site design. We found that binding sites tend to be shorter and fuzzier when they appear in promoter regions that bind multiple transcription factors. We further found that essential genes bind relatively fewer transcription factors, as do divergent promoters. We provide evidence that novel binding sites tend to appear in specific promoters that are already associated with multiple sites.

Conclusion

Two principal models may account for the observed correlations. First, it may be that the interaction between multiple factors compensates for the decreased specificity of each specific binding sequence. In such a scenario, binding-site fuzziness is a consequence of the presence of multiple binding sites. Second, binding sites may tend to appear in promoter regions that are subject to low selective pressure, which also allows for fuzzier motifs. The latter possibility may account for the relatively low number of binding sites found in promoters of essential genes and in divergent promoters.