Research
Protein-protein interactions of the hyperthermophilic archaeon Pyrococcus horikoshii OT3
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* Corresponding author: Harukazu Suzuki rgscerg@gsc.riken.jp
1 CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan
2 Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
3 Genome Science Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan
4 Highthroughput Factory, RIKEN Harima Institute, 1-1-1 Kouto, Mikazuki-cho, Sayo-gun, Hyogo 679-5148, Japan
5 Structural Biophysics Laboratory, RIKEN Harima Institute, 1-1-1 Kouto, Mikazuki-cho, Sayo-gun, Hyogo 679-5148, Japan
6 Division of Biomedical Engineering, Graduate School of Medicine, Kyushu University, Fukuoka 815-8582, Japan
Genome Biology 2005, 6:R98 doi:10.1186/gb-2005-6-12-r98
Published: 18 November 2005Additional files
Additional data file 1:
Detailed methods for the PPI mammalian two-hybrid system assay.
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Additional data file 2:
Experimental raw data are as follows: sheet 1, a list of 1,390 clones of P. horikoshii OT3 including results from the SOSUI program; sheet 2, a list of 980 clones that were used for the interaction assay in this study; sheet 3, a list of 170 primary protein interactions with their fold values for luciferase activity; sheet 4, a list of 107 selected protein interactions.
Format: XLS Size: 206KB Download file
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