Table 2 |
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|
20E-induced, EcR-dependent genes |
||||||
|
Gene |
20E |
20E primary |
EcRi -4 h |
EcRi 0 h |
EcRi 4 h |
Function |
|
|
||||||
|
Eip74A* |
11.0 |
12.6 |
-4.9 |
-4.5 |
-2.0 |
Transcription factor |
|
Drosomycin-2 |
8.1 |
-4.5 |
-7.3 |
-14.5 |
Defense response |
|
|
CG14664 |
4.7 |
-16.3 |
-22.9 |
n/o |
||
|
GST-E3 |
3.7 |
3.2 |
-1.9 |
Glutathione transferase |
||
|
Eip78C* |
3.6 |
3.3 |
-6.2 |
-6.9 |
-1.9 |
Nuclear receptor |
|
CG10444 |
3.4 |
-4.1 |
-7.1 |
-2.1 |
Na+-dependent multivitamin transporter |
|
|
Punch |
3.0 |
2.4 |
-3.1 |
-8.1 |
-2.2 |
GTP cyclohydrolase I |
|
CG5171 |
3.0 |
2.7 |
-1.7 |
-1.5 |
-3.5 |
Trehalose-phosphatase |
|
CG2444 |
3.0 |
-2.8 |
-2.4 |
2.3 |
n/o |
|
|
Cyp18a1* |
2.9 |
8.3 |
-2.3 |
-1.7 |
Cytochrome P450 |
|
|
CG5391 |
2.7 |
-5.2 |
3.1 |
n/o |
||
|
MESR3 |
2.6 |
-1.5 |
-3.0 |
-1.6 |
Ras signaling |
|
|
CG3714 |
2.6 |
2.3 |
-1.7 |
Nicotinate phosphoribosyltransferase |
||
|
rdgBbeta |
2.5 |
4.6 |
-2.2 |
-2.4 |
Phosphatidylinositol transporter |
|
|
CG8501 |
2.4 |
-1.9 |
-1.8 |
n/o |
||
|
l(3)00305 |
2.4 |
1.9 |
-1.6 |
Serine/threonine kinase |
||
|
CG11737 |
2.4 |
-2.3 |
-2.0 |
n/o |
||
|
Cabut |
2.3 |
5.9 |
-1.9 |
Transcription factor |
||
|
CG17834 |
2.3 |
1.6 |
-2.1 |
-4.4 |
n/o |
|
|
Sox box protein 14 |
2.3 |
2.9 |
-1.5 |
-1.7 |
Transcription factor |
|
|
JIL-1 |
2.2 |
-3.0 |
Protein serine/threonine kinase |
|||
|
CG18212 |
2.2 |
-2.1 |
-1.8 |
n/o |
||
|
CG13252 |
2.2 |
2.0 |
-2.1 |
-2.4 |
-2.7 |
n/o |
|
raspberry |
2.2 |
1.7 |
-1.9 |
-2.2 |
-1.8 |
IMP dehydrogenase |
|
supernumerary limbs |
2.2 |
-3.1 |
-2.6 |
Ubiquitin-protein ligase |
||
|
CG16995 |
2.1 |
1.5 |
-2.8 |
Defense response |
||
|
lamina ancestor |
2.1 |
2.9 |
-1.6 |
-3.1 |
-2.4 |
n/o |
|
CG33090 |
2.1 |
-3.9 |
-1.5 |
n/o |
||
|
CG7701 |
2.1 |
-1.6 |
n/o |
|||
|
rhea |
2.1 |
-1.9 |
-2.2 |
Actin binding |
||
|
Ptp52F |
2.1 |
2.2 |
-2.4 |
-1.6 |
Protein tyrosine phosphatase |
|
|
CG4822 |
2.1 |
1.4 |
-3.1 |
-2.2 |
ATPase activity |
|
|
big brain |
2.1 |
-2.4 |
Water channel |
|||
|
brain tumor |
2.0 |
2.9 |
-1.7 |
Translation regulator |
||
|
CG11529 |
2.0 |
-7.1 |
-2.2 |
2.0 |
Serine-type endopeptidase |
|
|
CG11509 |
2.0 |
1.9 |
-1.6 |
n/o |
||
|
CG3348 |
2.0 |
4.5 |
-3.0 |
-3.1 |
-1.7 |
n/o |
|
CG9989 |
2.0 |
-1.5 |
-2.2 |
-2.5 |
n/o |
|
|
blue cheese |
1.9 |
2.0 |
-2.0 |
-1.6 |
Intracellular protein transport |
|
|
CG14073 |
1.9 |
2.4 |
-1.5 |
Ankyrin-like |
||
|
kruppel homolog 1* |
1.9 |
3.5 |
-1.8 |
-2.2 |
Transcription factor |
|
|
CRMP |
1.8 |
2.6 |
-2.3 |
-3.2 |
Dihydropyrimidinase |
|
|
CG9801 |
1.8 |
3.1 |
-4.5 |
-5.7 |
n/o |
|
|
CG8483 |
1.8 |
5.4 |
-3.0 |
-2.8 |
Defense response |
|
|
CG9005 |
1.8 |
2.4 |
-1.8 |
-1.8 |
-1.8 |
Cell adhesion |
|
vrille |
1.6 |
3.5 |
-1.6 |
Transcription factor |
||
|
CG1342 |
1.6 |
2.4 |
-2.6 |
-8.5 |
Proteinase inhibitor I4, serpin |
|
|
DHR3* |
1.6 |
2.3 |
-2.9 |
Nuclear receptor |
||
|
Eip75B* |
1.5 |
2.8 |
-1.6 |
Nuclear receptor |
||
|
hairy |
4.4 |
-1.8 |
-1.6 |
Transcription factor |
||
|
CG9192 |
3.3 |
-1.7 |
n/o |
|||
|
CG5249 |
3.0 |
-1.5 |
Transcription factor |
|||
|
black |
2.7 |
-3.0 |
-7.0 |
Glutamate decarboxylase 2 |
||
|
CG2016 |
2.1 |
-2.0 |
-2.0 |
-2.0 |
n/o |
|
|
CG12539 |
2.1 |
-1.7 |
-1.6 |
Glucose dehydrogenase |
||
|
CG8788 |
2.0 |
-1.5 |
-2.5 |
-1.8 |
n/o |
|
|
|
||||||
|
Genes that show at least a twofold induction with either 20E alone (20E), or 20E + cycloheximide (20E primary) are listed in the order of their fold-induction by 20E alone. Downregulation of these genes upon EcR RNAi is shown for each time point, -4, 0, or 4 hours relative to puparium formation. Function is inferred from gene ontology on FlyBase. [40]. Asterisks denote previously identified 20E-regulated genes. n/o, no ontology. |
||||||
|
Beckstead et al. Genome Biology 2005 6:R99 doi:10.1186/gb-2005-6-12-r99 |
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