Table 10

Quantitative real-time PCR results for selected non-chromosome 21 genes in Table 2

Gene name

Chromosome

p value

Cerebrum

Cerebellum

Astrocyte

Heart


Microarray

qPCR

Microarray

qPCR

Microarray

qPCR

Microarray

qPCR


Myomesin 1 (skelemin) (185 kDa) (MYOM1)

18

8.82E-08

BBL

-

BBL

-

BBL

-

1.40

1.25 ± 0.06

Myoglobin (MB)

22

1.09E-07

BBL

-

BBL

-

BBL

-

1.64

1.58 ± 0.16

Calsequestrin 2 (cardiac muscle) (CASQ2)

1

1.56E-07

BBL

-

BBL

-

BBL

-

1.65

2.89 ± 0.58

Ras-related associated with diabetes (RRAD)

16

5.06E-06

BBL

-

BBL

-

BBL

-

0.49

0.82 ± 0.08

Troponin I, cardiac (TNNI3)

19

5.90E-06

BBL

-

BBL

-

BBL

-

1.62

1.31 ± 0.25

Insulin-like growth factor binding protein 7 (IGFBP7)

4

1.12E-05

1.41

1.02 ± 0.27

0.70

1.55 ± 0.33

1.74

2.12 ± 0.08

1.53

0.77 ± 0.09

Actin, alpha 1, skeletal muscle (ACTA1)

1

1.20E-05

BBL

-

BBL

-

BBL

-

4.17

6.27 ± 0.59

Calcineurin-binding protein calsarcin-1 (MYOZ2)

4

1.22E-05

BBL

-

BBL

-

BBL

-

1.49

1.35 ± 0.09

Cardiac troponin T2 (TNNT2)

1

2.56E-05

BBL

-

BBL

-

BBL

-

1.34

1.31 ± 0.25

A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8 (ADAMTS8)

11

3.21E-05

BBL

-

BBL

-

BBL

-

0.55

0.55 ± 0.17


Data were normalized to the HPRT housekeeping gene. P values were derived using the same ANOVA model used for the microarray data. Values for microarray data are fold-regulation. For each qPCR experiment, values were determined by measuring samples in duplicate at multiple concentrations (mean ± standard error). Each experiment was performed independently at least three times. BBL, below background level; qPCR, quantitative real-time PCR.

Mao et al. Genome Biology 2005 6:R107   doi:10.1186/gb-2005-6-13-r107

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