Figure 4.

Influence of mutations in the pheromone signaling pathway on translational responses of the HO transcript. The top of the gradient is to the left and the position of the 80S monosome is marked with the arrow. Percentage of total HO mRNA across polysome gradients in growing cells (filled circles) and cells treated with α-factor for 30 minutes (open circles) for strains (a) Δfus3 Δkss1, (b) Δste12, and (c) parental BY2125. Sucrose gradient centrifugation was performed and analyzed as described in Figure 2, using polymerase chain reaction primers that are common to all HO transcripts.