Table 1

Influence of pheromone treatment, nitrogen starvation and osmotic stress on 5' leader structure and ribosome loading

5' termini


Gene

Steady state

Treated

Loading ratio


Pheromone response

CRH1

-80

+2,+54

0.6

HO

-39

approx. -2,000

0.3

KAR5

+115,+166

-2

5.9

PRM2

+94,+297

-45

6.1

PRP39

-89

approx. +300

0.3

PRY3

-76

+452

0.6

SAG1

-826

-38

4.2

PRM4

-64

-64

1.9

BAR1 b

-52

-52

1.0a

FAR1 b

-47

-47

1.0a

STE2 b

-31

-31

1.0a

Nitrogen starvation

AMD2

-97

-23

2.7

ASP3

+657

-22

24.4

DAL5

-273

-53

15.0

DAL7

-159

-26

7.0

UGA1

-38

-38

2.0

MON1

-35

-35

1.8

ASP1 b

-41

-41

0.3

GDH1 b

-67

-67

0.7

Osmotic stress

AQY1

+28

-32

1.9

GCY1

-58

-58

2.6

PGM2

-60

-60

1.9


The 5' termini of the transcripts are expressed as nucleotides relative to the initiation codon of the open reading frame (ORF) and were determined by 5' rapid amplification of cDNA ends (RACE), except for HO and PRP39 in pheromone-treated cells, which were estimated from Northern blots and polymerase chain reaction walking. Ribosome loading is defined as the average number of ribosomes associated with a transcript and was determined as outlined in the method section except when indicated differently. The genes that exhibit a change in 5' untranslated region upon treatment are presented in bold font. The nitrogen starvation experiments were carried out with the △gcn2 strain. aThese values were calculated from the data presented by MacKay and coworkers [3]. bThese are control genes that do not change in ribosome loading.

Law et al. Genome Biology 2005 6:R111   doi:10.1186/gb-2005-6-13-r111

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