Split-plot ANOVA statistical design. Populations (ME, Maine; NJ, New Jersey; GA, Georgia) are treated as blocks, replicate individuals within each population (1, 2 and 3) as plots, and tissue (L, liver; H, heart; B, brain) within an individual as the split-plot factor. Nested within each tissue-by-individual sample are technical replicates including two dyes (Cy3 and Cy5) within each sample, two replicate hybridizations (A and B) per dye, and six replicate spots per hybridization. GM, grand mean.
Whitehead and Crawford Genome Biology 2005 6:R13 doi:10.1186/gb-2005-6-2-r13