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Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails

Hirofumi Kunitomo1, Hiroko Uesugi2, Yuji Kohara2 and Yuichi Iino1*

Author affiliations

1 Molecular Genetics Research Laboratory, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

2 Genome Biology Laboratory, National Institute of Genetics, Mishima 411-8540, Japan

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Citation and License

Genome Biology 2005, 6:R17  doi:10.1186/gb-2005-6-2-r17

Published: 31 January 2005

Abstract

It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method, in which poly(A) RNA was co-immunoprecipitated with an epitope-tagged poly(A)-binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory neuron-expressed genes.