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Open Access Highly Accessed Method

A universal method for automated gene mapping

Peder Zipperlen1*, Knud Nairz2*, Ivo Rimann2, Konrad Basler1, Ernst Hafen2, Michael Hengartner1 and Alex Hajnal2

Author Affiliations

1 Institute of Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

2 Institute of Zoology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

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Genome Biology 2005, 6:R19  doi:10.1186/gb-2005-6-2-r19

Published: 17 January 2005

Abstract

Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.