A universal method for automated gene mapping

doi:10.1186/gb-2005-6-2-r19

Genome Biology

Volume 6
Issue 2

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Zipperlen P
Nairz K
Rimann I
Basler K
Hafen E
Hengartner M
Hajnal A

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Nairz K
Rimann I
Basler K
Hafen E
Hengartner M
Hajnal A
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A universal method for automated gene mapping

Peder Zipperlen¹^* , Knud Nairz²^* , Ivo Rimann², Konrad Basler¹, Ernst Hafen², Michael Hengartner¹ and Alex Hajnal²

¹Institute of Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

²Institute of Zoology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

author email corresponding author email* Contributed equally

Genome Biology 2005, 6:R19doi:10.1186/gb-2005-6-2-r19


Published:	17 January 2005

Subject areas: Genetics, Molecular biology, Model organisms, Methods

Abstract

Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.