Open Access Highly Accessed Method

A universal method for automated gene mapping

Peder Zipperlen1*, Knud Nairz2*, Ivo Rimann2, Konrad Basler1, Ernst Hafen2, Michael Hengartner1 and Alex Hajnal2

Author Affiliations

1 Institute of Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

2 Institute of Zoology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

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Genome Biology 2005, 6:R19  doi:10.1186/gb-2005-6-2-r19

Published: 17 January 2005

Additional files

Additional data file 1:

General information on fly genetics

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Additional data file 2:

Proof-of-principle for chromosomal linkage with 3 known mutations on chromosome 2. Assays used to assess linkage were ZH1-01, ZH2-01, ZH3-05a, ZH4-03, ZH5-01 and ZHX-02

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Additional data file 3:

Mapping of let-23 to its subchromosomal region (C. elegans)

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Additional data file 4:

Mapping of rol-1 to its subchromosomal region (C. elegans)

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Additional data file 5:

Mapping of unc-52 to its subchromosomal region (C. elegans)

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Additional data file 6:

C. elegans FLP mapping flow chart

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Additional data file 7:

Drosophila FLP mapping flow chart

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Additional data file 8:

Electropherograms demonstrating the accuracy of allele-calling

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Additional data file 9:

Tables of primer and sequence data of experimentally verified FLP assays in C. elegans

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Additional data file 10:

Tables of primer and sequence data of experimentally verified FLP assays in Drosophila

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Additional data file 11:

A table of the refined genetic distances for FLP assays on the right arm of Drosophila chromosome 2

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Additional data file 12:

Additional non-validated FLPs (predicted C. elegans InDels LGI)

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Additional data file 13:

Additional non-validated FLPs (predicted C. elegans InDels LGII)

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Additional data file 14:

Additional non-validated FLPs (predicted C. elegans InDels LGIII)

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Additional data file 15:

Additional non-validated FLPs (predicted C. elegans InDels LGIV)

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Additional data file 16:

Additional non-validated FLPs (predicted C. elegans InDels LGV)

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Additional data file 17:

Additional non-validated FLPs (predicted C. elegans InDels LGX)

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Additional data file 18:

Additional non-validated FLPs (Drosophila)

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