FLP mapping in C. elegans. (a) Crossing scheme used to map mutations generated in the N2 Bristol background. The different classes of recombinants recovered in the F2 generation are shown. (b) Analysis of the zh41 mutation with Tier 1 assays establishes linkage to chromosome I. (c) Analysis with Tier 2 places zh41 between assays ZH1-01 and ZH1-15. ND, no data as a result of PCR reaction failure. (d) Ventral views of the vulva in wild-type and zh41 L4 larvae stained with the adherens junction antibody MH27 . In the wild type, the vulval cells have fused to generate the torroids that appear as concentric rings. zh41 mutants exhibit the same fusion defects observed in other lin-11 alleles .
Zipperlen et al. Genome Biology 2005 6:R19 doi:10.1186/gb-2005-6-2-r19