Table 1

Predicted G-protein-coupled receptor-like proteins in M. grisea
Known receptors used as query in BLAST against M. grisea proteins or another search method	M. grisea proteins retrieved by known receptor (BLASTP)	E-value	Other proteins homologous to M. grisea proteins retrieved by known receptor	PfamA GPCR domains (E-value)/conserved domain identified in the present study

Pheromone receptor (CAC86431; STE2-like)	MG04711.4*	3e-65		Pfam STE2 (2.1e-04)

Pheromone receptor STE3 (STE3_YEAST)	MG06452.4^†	2e-14		Pfam STE3 (1.1e-09)

cAMP receptor TASA (Q9NDL2)	MG06738.4* ^,†	5e-11		Pfam7tm_2 (1.3e-04)/cAMP_dom
			MG06797.4	cAMP_dom
			MG06257.4*	cAMP_dom

			MG00326.4	Pfam 7tm_2 (7.9e-05)/cAMP_dom

			MG00258.4	cAMP_dom

			MG10544.4	cAMP_dom

GPCR GPR1 (GPR1_YEAST)	MG08803.4	4e-18

GPCR STM1 (STM1_SCHPO)	MG04698.4*	5e-19		STM1_dom

	MG02855.4* ^,†	1e-17		STM1_dom

GPCR mPR (NP_848509)	MG05072.4*	6e-17		mPR_dom

			MG09091.4*	mPR_dom
			MG04679.4* ^,†	mPR_dom

PTH11 receptor (AF119670_1)	MG05871.4 (PTH11) * ^,†,‡	0		PTH11_dom

	MG10473.4^‡	3e-34		PTH11_dom
	MG06755.4^‡	1e-33		PTH11_dom
	MG07553.4^‡	2e-32		PTH11_dom
	MG09022.4* ^,‡	2e-27		PTH11_dom
	MG07565. * ^,†,‡4	6e-23		PTH11_dom
	MG07946.4^†,‡	3e-21		PTH11_dom

	MG11006.4	2e-32		PTH11_dom
	MG09070.4*	2e-29		PTH11_dom
	MG07806.4	2e-21		PTH11_dom
	MG03584.4^†	1e-22		PTH11_dom

	MG05214.4*	4e-31		PTH11_dom
	MG09863.4* ^,‡	1e-28		PTH11_dom

	MG10407.4*	3e-26		PTH11_dom

	MG10571.4* ^,†	4e-25		PTH11_dom

	MG01867.4^‡	1e-23		PTH11_dom

	MG09455.4^†,‡	2e-23		PTH11_dom
	MG10050.4^‡	1e-14		PTH11_dom
	MG09667.4	1E-22		PTH11_dom

	MG05352.4*	2e-22		PTH11_dom

	MG07420.4	1e-21		PTH11_dom

	MG10442.4	4e-20		PTH11_dom

	MG02160.4^†	6e-19		PTH11_dom

	MG02001.4* ^,†	1e-18		PTH11_dom

	MG10257.4	2e-18		PTH11_dom
	MG01905.4	2e-17		PTH11_dom
	MG07987.4	1e-16		PTH11_dom

	MG10438.4* ^,‡	6e-18		PTH11_dom

	MG06171.4*	1e-17		PTH11_dom

	MG07851.4	1e-17		PTH11_dom

	MG04935.4*	1e-17		PTH11_dom

	MG05386.4	3e-17		PTH11_dom

	MG09865.4* ^,†	3e-16		PTH11_dom

	MG09061.4	4e-16		PTH11_dom

	MG05514.4* ^,†	1e-16		PTH11_dom

	MG06535.4*	3e-14		PTH11_dom

	MG01190.4	7e-14		PTH11_dom

	MG10581.4*	7e-14		PTH11_dom

	MG03009.4*	2e-13		PTH11_dom

	MG10747.4	8e-13		PTH11_dom

	MG03935.4	2e-12		PTH11_dom
			MG04682.4*	PTH11_dom

	MG09416.4	1e-10		PTH11_dom

	MG02692.4*	2e-10		PTH11_dom

			MG07857.4	PTH11_dom

			MG00826.4	PTH11_dom

			MG06624.4* ^,†	PTH11_dom

			MG00435.4*	PTH11_dom

			MG08653.4*	PTH11_dom

			MG10706.4* ^,†	PTH11_dom

			MG04170.4*	PTH11_dom

			MG08525.4*	PTH11_dom

			MG00277.4*,†	PTH11_dom
			MG02365.4*	PTH11_dom

			MG06595.4	PTH11_dom

			MG06084.4*	PTH11_dom

			MG09437.4*	PTH11_dom

			MG01890.4	PTH11_dom

			MG01871.4	PTH11_dom

			MG03794.4	PTH11_dom

			MG01884.4*	PTH11_dom

InterProScan MG00532.1	MG00532.4 (weak similarity to animal GPCRs) *			Pfam 7tm_2 (1.4e-02)

Classes of GPCR-like protein in M. grisea were subdivided on the basis of BLASTP analysis and shared domains, as described in Materials and methods. They were clustered into paralogous families if the proteins showed 30% identity and 80% overlap over the complete length of the protein. Paralogous families are separated by a blank line. The GPCR-like proteins in M. grisea could be classified into nine subclasses containing more than one member and 48 containing a single member. Six subclasses contained two members, two contained three and one contained six. *M. grisea proteins represented by genes expressed in microarray experiments. ^†M. grisea proteins that are represented in M. grisea ESTs. ^‡Proteins containing the cysteine-rich CFEM domain.
Kulkarni et al. Genome Biology 2005 6:R24 doi:10.1186/gb-2005-6-3-r24