Figure 10.

ORFs with low and high GC3 have different codon-pair contexts. To highlight the effect of GC3 bias on codon-pair context, the context maps for the subgroups of low GC3 and high GC3 ORFs of both S. cerevisiae and E. coli were overlapped using the differential display codon-pair context (DCM) tool. The DCM maps for S. cerevisiae and E. coli showed significant differences (light blue cells in the DCMs), in particular in E. coli, indicating that GC3 bias influences codon-pair context.