Table 1

Quantitative RT-PCR confirmation of GeneChip Microarray data for genes 13903_at (At3g54050) and 17392_s_at (At3g53260) in Col-0 and C24

Samples

13903_at

17392_s_at



log2(rRHI)

log2(nRHI)

Taqman

log2(rRHI)

log2(nRHI)

Taqman

Col-0-4 day seedlings

10.11940591

0.911529477

1.348 ± 0.262

10.38351776

0.658285681

0.362 ± 0.024

Col-0-2 week leaf

11.80337083

2.595494397

4.652 ± 0.389

10.56878747

0.84355539

0.299 ± 0.050

Col-0-11 week leaf

10.77324577

1.565369327

1.415 ± 0.336

10.33789612

0.612664042

0.163 ± 0.052

Col-0-2 week root

7.674725423

-1.533151014

0.134 ± 0.014

11.26384894

1.538616864

1.313 ± 0.324

Col-0-5 week root

7.873250697

-1.334625741

0.590 ± 0.064

10.99787749

1.272645415

0.648 ± 0.246

Col-0-influorescence

10.09145865

0.883582211

1.320 ± 0.247

11.01034472

1.285112643

0.519 ± 0.104

Col-0-flower

10.42134176

1.213465325

2.093 ± 0.658

10.62442631

0.899194238

0.263 ± 0.053

Col-0-young siliques

10.65287316

1.444996723

1.999 ± 2.885

10.57630495

0.851072873

0.430 ± 0.197

Col-0-mature siliques

9.475076913

0.267200476

1.432 ± 2.345

10.80990555

1.084673476

0.473 ± 0.113

C24-4 day seedlings

10.90593269

1.883371001

3.690 ± 0.482

10.20742445

0.596353845

0.321 ± 0.059

C24-2 week leaf

12.29789156

3.275329874

6.819 ± 3.507

10.65702025

1.04594965

0.299 ± 0.044

C24-11 week leaf

12.09006973

3.067508045

6.073 ± 1.283

9.19787898

-0.413191622

0.071 ± 0.037

C24-2 week root

7.550943148

-1.471618541

0.069 ± 0.022

10.89199181

1.280921209

0.790 ± 0.133

C24-5 week root

7.945743693

-1.076817996

0.317 ± 0.087

11.16598953

1.554918929

1.122 ± 0.324

C24-influorescence

10.72350042

1.700938727

2.397 ± 0.304

11.10540542

1.494334819

0.743 ± 0.105

C24-flower

10.71423996

1.691678266

1.054 ± 0.167

9.761854806

0.150784204

0.153 ± 0.048

C24-young siliques

11.01401689

1.991455197

1.885 ± 0.726

10.61478826

1.00371766

0.365 ± 0.058

C24-mature siliques

11.21144986

2.188888168

3.808 ± 0.569

11.24013223

1.629061624

1.002 ± 0.151

Correlation with log2 (Taqman assay)

0.925

0.933

0.801

0.821


gDHI for 13903_at is 591.35 and 520.07 for Col-0 and C24, respectively. gDHI for 17392_s_at is 846.42 and 782.02 for Col-0 and C24, respectively.

Chen et al. Genome Biology 2005 6:R32   doi:10.1186/gb-2005-6-4-r32

Open Data