Open Access Method

Pilot Anopheles gambiae full-length cDNA study: sequencing and initial characterization of 35,575 clones

Shawn M Gomez1, Karin Eiglmeier1, Beatrice Segurens2, Pierre Dehoux3, Arnaud Couloux2, Claude Scarpelli2, Patrick Wincker2, Jean Weissenbach2, Paul T Brey1 and Charles W Roth1*

  • * Corresponding author: Charles W Roth croth@pasteur.fr

  • † Equal contributors

Author Affiliations

1 Unité de Biochimie et Biologie Moléculaire des Insectes and CNRS FRE 2849, Institut Pasteur, 75724 Paris Cedex 15, France

2 Genoscope/Centre National de Séquençage and CNRS UMR 8030, 91057 Evry Cedex, France

3 Plate-forme Intégration et Analyse Génomiques, Institut Pasteur, 75724 Paris Cedex 15, France

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Genome Biology 2005, 6:R39  doi:10.1186/gb-2005-6-4-r39

Published: 15 March 2005

Abstract

We describe the preliminary analysis of over 35,000 clones from a full-length enriched cDNA library from the malaria mosquito vector Anopheles gambiae. The clones define nearly 3,700 genes, of which around 2,600 significantly improve current gene definitions. An additional 17% of the genes were not previously annotated, suggesting that an equal percentage may be missing from the current Anopheles genome annotation.