Functional analysis of human and chimpanzee promoters

Florian Heissig , Johannes Krause , Jaroslaw Bryk , Philipp Khaitovich , Wolfgang Enard and Svante Pääbo

Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D-04103 Leipzig, Germany

author email corresponding author email

Genome Biology 2005, 6:R57doi:10.1186/gb-2005-6-7-r57


Published:	1 July 2005

Subject areas: Model organisms, Molecular biology

Additional files

Additional File 1:

Supplementary Table 1: Listed are all 71 genes that were identified as being at least 1.4-fold differently expressed in liver and/or brain. For the probe sets the change p-values (MAS 5.0) averaged over the 36 pairwise comparisons between human and chimpanzee samples [6] and the signal log₂ratio (slr; MAS 5.0) for the probe sets are given. p-values close to 1 suggest a higher expression in chimpanzees, as does a negative slr.

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Additional File 2:

Supplementary Table 2: The primer sequences used to amplify the promoter fragments are given here. Primer sequences are written 5' to 3' and lower-case letters indicate added restriction sites.

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Additional File 3:

Supplementary Table 3: The normalized promoter activities are listed; that is, the measured luciferase activity of the promoter constructs divided by the sea-pansy luciferase activity, for each of the three replicates that were done for each of the three clones for humans and chimpanzees in the neuroblastoma cell line and the cervix carcinoma cell line, respectively, for the 12 genes analyzed.