Open Access Method

Analysis of the Macaca mulatta transcriptome and the sequence divergence between Macaca and human

Charles L Magness1, P Campion Fellin1, Matthew J Thomas2, Marcus J Korth2, Michael B Agy3, Sean C Proll2, Matthew Fitzgibbon2, Christina A Scherer1, Douglas G Miner1, Michael G Katze2,3 and Shawn P Iadonato1*

Author Affiliations

1 Illumigen Biosciences Inc., Suite 450, 2203 Airport Way South, Seattle, WA 98134, USA

2 Department of Microbiology, University of Washington, Seattle, WA 98195-8070, USA

3 Washington National Primate Research Center, University of Washington, Seattle, WA 98195-8070, USA

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Genome Biology 2005, 6:R60 doi:10.1186/gb-2005-6-7-r60

Published: 30 June 2005

Abstract

We report the initial sequencing and comparative analysis of the Macaca mulatta transcriptome. Cloned sequences from 11 tissues, nine animals, and three species (M. mulatta, M. fascicularis, and M. nemestrina) were sampled, resulting in the generation of 48,642 sequence reads. These data represent an initial sampling of the putative rhesus orthologs for 6,216 human genes. Mean nucleotide diversity within M. mulatta and sequence divergence among M. fascicularis, M. nemestrina, and M. mulatta are also reported.