Open Access Method

Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray

Mark G Carter1, Alexei A Sharov1, Vincent VanBuren1, Dawood B Dudekula1, Condie E Carmack2, Charlie Nelson2 and Minoru SH Ko1*

Author Affiliations

1 Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Baltimore, MD 21224, USA

2 Agilent Technologies, Deer Creek Rd, Palo Alto, CA 94304, USA

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Genome Biology 2005, 6:R61 doi:10.1186/gb-2005-6-7-r61

Published: 30 June 2005

Abstract

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.