Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray
1 Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Baltimore, MD 21224, USA
2 Agilent Technologies, Deer Creek Rd, Palo Alto, CA 94304, USA
Genome Biology 2005, 6:R61 doi:10.1186/gb-2005-6-7-r61Published: 30 June 2005
The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.