Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray

doi:10.1186/gb-2005-6-7-r61

Genome Biology

Volume 6
Issue 7

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VanBuren V
Dudekula DB
Carmack CE
Nelson C
Ko MS

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Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray

Mark G Carter¹ , Alexei A Sharov¹ , Vincent VanBuren¹ , Dawood B Dudekula¹ , Condie E Carmack² , Charlie Nelson² and Minoru SH Ko¹

¹Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Baltimore, MD 21224, USA

²Agilent Technologies, Deer Creek Rd, Palo Alto, CA 94304, USA

author email corresponding author email

Genome Biology 2005, 6:R61doi:10.1186/gb-2005-6-7-r61


Published:	30 June 2005

Subject areas: Genome studies, Methods

Abstract

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.