Figure 1.

60-mer oligonucleotide probe linearity testing. To test the performance of 21,044 60-mer oligonucleotide probes, E12.5 embryo RNA and placenta RNA were combined to form five pairs of duplicate samples containing from 0 to 100% placental RNA. Box-plot distribution data for each placental RNA input level is shown above, with median values labeled. The boxes show the 25-75 percentile range, with the mean and median indicated by the central straight line and diamond, respectively. Upper and lower bars show the 2.5 to 97.5 percentile range. Observed fraction medians are within 0.075 of input values, and 95% of values are within 0.405 of input values.