Table 3

qPCR primer pairs used to quantitate endogenous transcripts in this study
Gene symbol
 Forward/reverse qPCR oligo sequence
 Optimal concentration
 Amplicon
 Intron spanned?







Size
 Tm
Ank
 5'-AGTACCATAGTACACTCGGTTACCTGTCCTG-3'
 900 nM
 114 bp
 78.8°C
 Yes

5'-GCAAAGCTTTAAGTCGTAATCTAGCATCC-3'
 50 nM








Axl/Ufo
 5'-CGACTACCTGCGTCAAGGAAATCG-3'
 300 nM
 112 bp
 82.8°C
 Yes

5'-AAAACTTGGCCGGTCTCGAGG-3'
 300 nM








Cd34
 5'-TGCTCTGGAATCCGAGAAGTGAGG-3'
 300 nM
 140 bp
 78.0°C
 Yes

5'-TCAGCCTCAGCCTCCTCCTTTTC-3'
 300 nM








Dnchc1
 5'-AACTAAACCCAGCCATTCGGCC-3'
 300 nM
 98 bp
 84.3°C
 No

5'-TTGCGTTGGCGGGTGACAG-3'
 900 nM








Gap43
 5'-GAGAAGGGAAGGAGAGAAGGCAGG-3'
 900 nM
 131 bp
 79.5°C
 Yes

5'-TCCGGCTTGACACCATCTTGTTC-3'
 900 nM








Gapd
 5'-CGGAGTCAACGGATTTGGTCGTAT-3'
 900 nM
 214 bp
 82.6°C
 Yes

5'-GAAGATGGTGATGGGCTTCC-3'
 300 nM








H19
 5'-AGCTAACACTTCTCTGCTGCTCTCTGG-3'
 300 nM
 144 bp
 81.4°C
 Yes

5'-ATCTTCTTGATTCAGAACGAGACGGAC-3'
 900 nM








Hand1
 5'-GAGATGTATACCTGAGAGCAACAGGCATGATAGGTAG-3'
 300 nM
 113 bp
 75.1°C
 No

5'-CTTCTCCTTCATTTCTTTCCTTTTCCTTC-3'
 900 nM








Hif1a
 5'-GTCAGCAGTACATGGTAGCCACAATTG-3'
 900 nM
 139 bp
 74.4°C
 No

5'-GATCCAGGCTTAACAATTCCATAGGC-3'
 300 nM








Hmga1
 5'-AATTCAGGAGGATGAACATCTGACGC-3'
 900 nM
 114 bp
 77.3°C
 No

5'-TCTGTTCACAAACTACCTCTGGACGG-3'
 50 nM








Hprt1
 5'-AACAATGCAAACTTTGCTTTCCCTG-3'
 300 nM
 123 bp
 80.1°C
 Yes

5'-TCAAATCCAACAAAGTCTGGCCTG-3'
 300 nM








Igf2bp3
 5'-AAGTATACATTCTCACAGAGACAGGATCGAGTGACTG-3'
 900 nM
 126 bp
 81.5°C
 No

5'-AAAGACAGATTTGCTTAACCAACAGACG-3'
 900 nM








Lpl
 5'-TTTCCAGCCAGGATGCAACATTG-3'
 300 nM
 105 bp
 82.3°C
 No

5'-TGAATGGAGCGCTCATGCGAG-3'
 900 nM








Myo1b
 5'-AATACACACCTTGTACCAATCAGCTCTCTC-3'
 900 nM
 143 bp
 76.1°C
 No

5'-TGATAAGAAGAGGCTGAGAGCCGTTC-3'
 900 nM


Carter et al. Genome Biology 2005 6:R61   doi:10.1186/gb-2005-6-7-r61

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