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Open Access Method

Genomic analysis of heat-shock factor targets in Drosophila

Ian Birch-Machin1, Shan Gao2, David Huen2, Richard McGirr1, Robert AH White1 and Steven Russell2*

Author Affiliations

1 Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK

2 Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK

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Genome Biology 2005, 6:R63  doi:10.1186/gb-2005-6-7-r63

Published: 10 June 2005

Abstract

We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.