Figure 2.

Comparison of miR-150 expression by arrays and Northern blotting. (a) Array data show that miR-150 (arrows) is highly expressed in spleen B and naïve T cells, but not in pro-B cells or fully differentiated Th1 and Th2 clones. (b) Northern blot analysis for miR-150 in various lymphoid and non-lymphoid tissues and cell types. U6 RNA levels are shown as loading control. (c) Northern blots of different cell types unstimulated or stimulated for the indicated amounts of time with either PMA and ionomycin (BMMC) or anti-CD3 and anti-CD28 (Th1 and Th2 primary cells). Preliminary data obtained both in Northern blot and arrays showed no difference in miRNA expression between BMMC left untreated or treated with cyclosporin A (CsA) (not shown). (d) Ethidium bromide staining of gel of total RNA from samples used in Figure 2c, showing equivalent RNA amounts. BMMC, bone marrow-derived mast cells; MEF, mouse embryo fibroblast.