Figure 6.

Δydr065w mutants are defective for vacuolar acidification. Wild-type (BY4743) and BY4743 Δydr065w cells were harvested in exponential phase, incubated with LysoSensor Green DND-189, and then examined by differential interference contrast (DIC) (left panel) and fluorescence (right panel) microscopy. Failure to accumulate the fluorophore indicates defective vacuolar acidification. Intact vacuoles in the mutant cells are apparent in the DIC image.