Table 3

Final concentration of elements in growth medium

Element

Form added

Supplemented concentration

Final concentration


Calcium

CaCl2

1 mM

1.2 mM

Cobalt

CoCl2

5 μM

5.1 μM

Copper

CuCl2

100 μM

110 μM

Iron

-

-

19 μM

Magnesium

-

-

490 μM

Manganese

MnCl2

10 μM

11 μM

Nickel

NiCl2

125 μM

160 μM

Potassium

-

-

12 mM

Phosphorus

-

-

5.7 mM

Selenium

Na2SeO3

75 μM

75 μM

Sodium

-

-

16 mM

Sulfur

-

-

5.4 mM

Zinc

ZnCl2

100 μM

160 μM


Calcium, cobalt, copper, manganese, nickel, selenium and zinc were added to rich YPD medium (1% yeast extract, 2% peptone, 2% glucose) to facilitate their detection in cells by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). These supplemented levels did not exceed 10% of the minimal growth inhibitory concentration determined for this strain (data not shown). The final concentration of these elements in the growth medium measured by ICP-AES is also shown and represents the supplemented levels plus those present in the YPD medium alone. The same lots of all medium components (such as yeast extract, peptone) were used throughout this study to maintain consistent growth conditions. Boron was added as H2BO3 at 181 μM and molybdenum was added as NaMoO4 at 10 μM. Despite this supplementation, levels of these two minerals accumulated by cells remained below the level of detection. -, not supplemented.

Eide et al. Genome Biology 2005 6:R77   doi:10.1186/gb-2005-6-9-r77

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