Research
Loss of LIN-35, the Caenorhabditis elegans ortholog of the tumor suppressor p105Rb, results in enhanced RNA interference
-
* Corresponding author: Andrew G Fraser agf@sanger.ac.uk
1 The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
2 Department of Biological Sciences, Columbia University, New York, NY 10027, USA
Genome Biology 2006, 7:R4 doi:10.1186/gb-2006-7-1-r4
Published: 20 January 2006Additional files
Additional data file 1:
The complete set of genes screened by RNAi feeding.
Format: TXT Size: 88KB Download file
Additional data file 2:
Genes that have an enhanced RNAi phenotype in each of the hypersensitive strains or in the non-hypersensitive strain efl-1(se1) are indicated. Genes with a weak RNAi phenotype in wild-type (N2) worms are also indicated, together with the RNAi phenotype in lin-35(n745) animals. Emb, embryonic lethal; Gro, growth arrest of parental generation resulting in an F1 brood size of 0; Red, reduced brood size; Ste, sterile.
Format: TXT Size: 3KB Download file
Additional data file 3:
Genes with nonviable RNAi phenotypes in lin-35(n745) that also have nonviable null phenotypes.
Format: DOC Size: 28KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional data file 4:
Genes from chromosome III tested for enhanced RNAi phenotypes in the strains lin-35(n745) and rrf-3(pk1426). Gene 'functional class' descriptions are taken from [4].
Format: TXT Size: 37KB Download file
Additional data file 5:
682 RNAi clones targeting genes from the start of chromosome III were fed to lin-35(n745), rrf-3(pk1426) and wild-type worms. Forty-two clones had an RNAi phenotype that was stronger in lin-35(n745) than in rrf-3(pk1426). Of these, 11 had an RNAi phenotype in rrf-3 that was further enhanced in lin-35. 0, no visible phenotype; Adl, adult lethal; Emb, embryonic lethal (scored on a semi-quantitative scale from 0 to 3); Gro, growth arrest in first generation; Lvl, larval lethal in first generation; Red, reduced brood size; Ste, sterile.
Format: TXT Size: 2KB Download file
Additional data file 6:
Strain PD6249 expresses a myo-3::GFP transgene in all muscle cells. Expression of the transgene is partially silenced at 20°C due to a mutation in the gene tam-1 [25]. RNAi against (a) a control gene, or (b) dcr-1 has no effect on the level of transgene silencing (in contrast to silencing resulting from loss of lin-35, which is lost if components of the RNAi pathway are inactivated; Figure 2).
Format: PDF Size: 69KB Download file
This file can be viewed with: Adobe Acrobat Reader