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Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes

Rammohan Narayanaswamy, Wei Niu, Alexander D Scouras, G Traver Hart, Jonathan Davies, Andrew D Ellington, Vishwanath R Iyer* and Edward M Marcotte*

Author Affiliations

Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, 2500 Speedway, University of Texas, Austin, TX 78712, USA

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Genome Biology 2006, 7:R6  doi:10.1186/gb-2006-7-1-r6

Published: 31 January 2006

Abstract

We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed, stained for subcellular features, then imaged via automated, high-throughput microscopy, allowing systematic phenotypic characterization. We used this technology to identify genes involved in the response of yeast to mating pheromone. Besides morphology assays, cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays, enabling new classes of genetic assays based on cell imaging.