Table 3

Comparison of gold and silver standard families to Pfam and SCOP families

Gold/silver standard family
Pfam family*
SCOP family*
Reaction catalyzed

Enolase
enolase_n, enolase_c
Enolase N-terminal domain-like, enolase
Dehydration of 2-phospho-D-glycerate
Methylaspartate ammonia-lyase
maal_n, maal_c
Enolase N-terminal domain-like, D-glucarate dehydratase-like
Elimination of ammonia from methylaspartic acid
Mandelate racemase
mr_mle_n, mr_mle
Enolase N-terminal domain-like, D-glucarate dehydratase-like
Racemization of S-mandelate to R-mandelate
Dipeptide epimerase
mr_mle_n, mr_mle
Enolase N-terminal domain-like, D-glucarate dehydratase-like
Dipeptide epimerization
Chloromuconate cycloisomerase
mr_mle_n, mr_mle
Enolase N-terminal domain-like, D-glucarate dehydratase-like
Chloromuconate lactonization
Muconate cycloisomerase
mr_mle_n, mr_mle
Enolase N-terminal domain-like, D-glucarate dehydratase-like
Muconate lactonization
Ortho-succinylbenzoate synthase
mr_mle_n, mr_mle
Enolase N-terminal domain-like, D-glucarate dehydratase-like
Dehydration of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid
Glucarate dehydratase
mr_mle_n, mr_mle
Enolase N-terminal domain-like, D-glucarate dehydratase-like
Dehydration of D-glucarate
Galactonate dehydratase
mr_mle_n, mr_mle
NA
Dehydration of D-galactonate
Fosfomycin resistance protein FosA
Glyoxalase
Antibiotic resistance proteins
Addition of glutathione to the oxirane ring of fosfomycin
2,3-Dihydroxybiphenyl dioxygenase
Glyoxalase
Extradiol dioxygenases
Extradiol cleavage of 2,3-dihydroxybiphenyl to 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
3,4-Dihydroxyphenylacetate 2,3-dioxygenase
Glyoxalase
Extradiol dioxygenases
extradiol cleavage of 3,4-dihydroxyphenylacetate to 2-hydroxy-5-carboxymethylmuconate semialdehyde
3-Methylcatechol 2,3-dioxygenase
Glyoxalase
Extradiol dioxygenases
Extradiol cleavage of 3-methylcatechol to 2-hydroxy-6-oxo-2,4-heptadienoate
4-Hydroxyphenylpyruvate dioxygenase
Glyoxalase
Extradiol dioxygenases
Conversion of 4-hydroxyphenylpyruvate to homogentisate
Glyoxalase I
Glyoxalase
Glyoxalase I
Conversion of methylglyoxal (hemithioacetal form) to S-D-lactoylglutathione
Methylmalonyl-CoA epimerase
Glyoxalase
Methylmalonyl-CoA epimerase
Epimerization of (2R)-methylmalonyl-CoA to (2S)-methylmalonyl-CoA
1,2-Dihydroxynaphthalene dioxygenase
Glyoxalase
NA
Extradiol cleavage of 1,2-dihydroxynaphthalene
2,2',3-Trihydroxybiphenyl dioxygenase
Glyoxalase
NA
Extradiol cleavage of 2,2',3-trihydroxybiphenyl to 2-hydroxy-6-oxo-(2-hydroxyphenyl)-hexa-2,4-dienoic acid
2,3-Dihydroxy-p-cumate-3,4-dioxygenase
Glyoxalase
NA
Extradiol cleavage of 2,3-dihydroxy-p-cumate to 2-hydroxy-3-carboxy-6-oxo-7-methylocta-2,4-dienoate
2,4,5-Trihydroxytoluene oxygenase
Glyoxalase
NA
Extradiol cleavage of 2,4,5-trihydroxytoluene
2,6-Dichlorohydroquinone dioxygenase
Glyoxalase
NA
Extradiol cleavage of 2,6-dichlorohydroquinone
3-Isopropylcatechol-2,3-dioxygenase
Glyoxalase
NA
Extradiol cleavage of 3-isopropylcatechol
4-Hydroxymandelate synthase
Glyoxalase
NA
Conversion of p-hydroxyphenylpyruvate to L-p-hydroxymandelate
Catechol 2,3-dioxygenase
Glyoxalase
NA
Extradiol cleavage of catechol to alpha-hydroxymuconic semialdehyde
Fosfomycin resistance protein FosB
Glyoxalase
NA
Addition of L-cysteine to the oxirane ring of fosfomycin
Fosfomycin resistance protein FosX
Glyoxalase
NA
Addition of water to the oxirane ring of fosfomycin
Adenosine deaminase
A_deaminase
Adenosine deaminase (ADA)
Deamination of adenosine
AMP deaminase
A_deaminase
NA
Deamination of AMP
Cytosine deaminase
Amidohydro_1
Cytosine deaminase catalytic domain; cytosine deaminase
Deamination of cytosine
N-acyl-D-amino-acid deacylase
Amidohydro_1
D-aminoacylase, catalytic domain; D-aminoacylase
Hydrolysis of an N-acyl-D-amino-acid
Dihydroorotase3
Amidohydro_1
Dihydroorotase
Synthesis of dihydroorotate from carbamoyl aspartate
D-hydantoinase
Amidohydro_1
Hydantoinase (dihydropyrimidinase), catalytic domain; hydantoinase (dihydropyrimidinase)
Hydrolytic ring cleavage of a dihydropyrimidine
L-hydantoinase
Amidohydro_1
Hydantoinase (dihydropyrimidinase), catalytic domain; hydantoinase (dihydropyrimidinase)
Hydrolytic ring cleavage of a 5 membered cyclic diamide
Isoaspartyl dipeptidase
Amidohydro_1
Isoaspartyl dipeptidase, catalytic domain; isoaspartyl dipeptidase
Hydrolysis of beta-l-isoaspartyl linkage of a dipeptide
Adenine deaminase
Amidohydro_1
NA
Deamination of adenine
Allantoinase
Amidohydro_1
NA
Hydrolysis of allantoin
Ammelide aminohydrolase
Amidohydro_1
NA
Deamination of ammelide
Aryldialkylphosphatase
Amidohydro_1
NA
Hydrolysis of an organophosphate
Atrazine chlorohydrolase
Amidohydro_1
NA
Hydrolytic dechlorination of atrazine
Dihydroorotase1
Amidohydro_1
NA
Synthesis of dihydroorotate from carbamoyl aspartate
Dihydroorotase2
Amidohydro_1
NA
Synthesis of dihydroorotate from carbamoyl aspartate
Guanine deaminase
Amidohydro_1
NA
Deamination of guanine
Hydroxydechloroatrazine ethylaminohydrolase
Amidohydro_1
NA
Conversion of 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine to N-isopropylammelide
Imidazolonepropionase
Amidohydro_1
NA
Hydrolysis of (S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate
Melamine deaminase
Amidohydro_1
NA
Deamination of melamine
N-acetylgalactosamine-6-phosphate deacetylase
Amidohydro_1
NA
Deacetylation of N-acetylgalactosamine-6-phosphate
N-isopropylammelide isopropylaminohydrolase
Amidohydro_1
NA
Conversion of N-isopropylammelide to isopropylamine
S-triazine hydrolase
Amidohydro_1
NA
Hydrolysis of a triazine
Trzn
Amidohydro_1
NA
Hydrolysis of a triazine
N-acetylglucosamine-6-phosphate deacetylase
Amidohydro_1
N-acetylglucosamine-6-phosphate deacetylase, catalytic domain; N-acetylglucosamine-6-phosphate deacetylase
Deacetylation of N-acetylglucosamine-6-phosphate
Urease
Amidohydro_1, urease
Alpha-subunit of urease, catalytic domain; alpha-subunit of urease; urease, beta-subunit; urease, gamma-subunit
Hydrolysis of urea to ammonia and carbon dioxide
1-Aminocyclopropane-1-carboxylate deaminase
None
NA
Deamination of 1-aminocyclopropane-1-carboxylate
Phosphotriesterase
PTE
Phosphotriesterase-like
Hydrolysis of an organophosphate
Membrane dipeptidase
Renal_dipeptase
Renal dipeptidase
Hydrolysis of a dipeptide
Glucuronate isomerase
UxaC
Uronate isomerase TM0064
Conversion of D-glucuronate to D-frucuronate
Delta(3,5)-delta(2,4)-dienoyl-CoA isomerase
ECH
Crotonase-like
Isomerization of 3,5-dienoyl-CoA to 2,4-dienoyl-CoA
Methylmalonyl-CoA decarboxylase
ECH
Crotonase-like
Decarboxylation of methylmalonyl CoA
Methylglutaconyl-CoA hydratase
ECH
Crotonase-like
Hydration of 3-methylglutaconyl-CoA
Enoyl-CoA hydratase
ECH
Crotonase-like
Hydration of trans-2-enoyl-CoA thiolester
4-Chlorobenzoate dehalogenase
ECH
Crotonase-like
Hydrolytic dehalogenation of 4-chlorobenzoyl-CoA
Dodecenoyl-CoA delta-isomerase (peroxisomal)
ECH
Crotonase-like
Isomerization of 3-enoyl-CoA to 2-enoyl-CoA
Methylglutaconyl-CoA hydratase 2
ECH
NA
Hydration of 3-methylglutaconyl-CoA
Histone acetyltransferase
ECH
NA
Acetylation of histone
2-Ketocyclohexanecarboxyl-CoA hydrolase
ECH
NA
Cleavage of 2-ketocyclohexanecarboxyl-CoA to pimelyl-CoA
1,4-Dihydroxy-2-napthoyl-CoA synthase
ECH
NA
Cyclization of o -succinylbenzoate-CoA thioester
Feruloyl-CoA hydratase/lyase
ECH
NA
Hydration and nonoxidative cleavage of feruloyl-SCoA to vanillin and acetyl-SCoA
Crotonobetainyl-CoA hydratase
ECH
NA
Hydration of crotonobetainyl-CoA
Cyclohex-1-enecarboxyl-CoA hydratase
ECH
NA
Hydration of cyclohex-1-enecarboxyl-CoA
Cyclohexa-1,5-dienecarbonyl-CoA hydratase
ECH
NA
Hydration of cyclohexa-1,5-diene-1-carboxyl-CoA
3-Hydroxyisobutyryl-CoA hydrolase
ECH
NA
Hydrolysis of 3-hydroxyisobutyryl-CoA
Dodecenoyl-CoA delta-isomerase (mitochondrial)
ECH
NA
Isomerization of 3-enoyl-CoA to 2-enoyl-CoA
Beta-phosphoglucomutase
Hydrolase
Beta-phosphoglucomutase-like
Conversion of beta-glucose-1-phosphate to glucose-6-phosphate
P-type ATPase
Hydrolase
Calcium ATPase, catalytic domain P
Dephosphorylation of ATP to ADP
Epoxide hydrolase N-terminal phosphatase
Hydrolase
Epoxide hydrolase, N-terminal domain
Dephosphorylation
2-Haloacid dehalogenase
Hydrolase
L-2-haloacid dehalogenase, HAD
Dehalogenation of (s)-2-haloacid
Phosphoserine phosphatase
Hydrolase
Phosphoserine phosphatase
Dephosphorylation of phosphoserine
Phosphonoacetaldehyde hydrolase
Hydrolase
Phosphonoacetaldehyde hydrolase
Hydrolysis of phosphonoacetaldehyde
2-Deoxyglucose-6-phosphatase
Hydrolase
NA
Dephosphorylation of 2-deoxyglucose-6-phosphate
Phosphoglycolate phosphatase
Hydrolase
NA
Dephosphorylation of 2-phosphoglycolate
Phosphoglycolate phosphatase 2
Hydrolase
NA
Dephosphorylation of 2-phosphoglycolate
Glycerol-3-phosphate phosphatase
Hydrolase
NA
Dephosphorylation of glycerol-3-phosphate
Pyridoxal phosphatase
Hydrolase
NA
Dephosphorylation of pyridoxal 5'-phosphate
Enolase-phosphatase
Hydrolase
NA
Oxidative cleavage
Histidinol-phosphatase
IGPD
NA
Dephosphorylation of L-histidinol-phosphate
Sucrose-phosphatase
S6PP
NA
Dephosphorylation of sucrose 6-phosphate
Trehalose-phosphatase
Trehalose_ PPase
NA
Dephosphorylation of trehalose 6-phosphate
5'-Nucleotidase
None
5' (3')-Deoxyribonucleotidase (dNT-2)
Dephosphorylation of 5' nucleotide
Deoxy-D-mannose-octulosonate 8-phosphate phosphatase
None
Probable phosphatase YrbI
Dephosphorylation of 3-deoxy-D-manno-octulosonate 8-phosphate
Polynucleotide 5'-hydroxyl-kinase carboxy-terminal phosphatase
None
Polynucleotide kinase, phosphatase domain
Dephosphorylation of 3' nucleotide
mdp-1
None
NA
Dephosphorylation
Mannosyl-3-phosphoglycerate phosphatase
None
NA
Dephosphorylation of 2(alpha-D-mannosyl)-3-phosphoglycerate

*Some gold standard families correspond to multiple Pfam and/or SCOP families because Pfam and SCOP divide the enzymes in question into multiple structural domains, each with a different family assignment. NA = Not applicable, IGPD, Pfam Imidazoleglycerol-phosphate dehydratase family; ECH, Pfam Enoyl-CoA hydratase/isomerase family; PTE, Pfam Phosphotriesterase family.

Brown et al. Genome Biology 2006 7:R8   doi:10.1186/gb-2006-7-1-r8