Table 3

PA14-specific regions containing genes required for C. elegans killing

PA14 region ID

Number of genes in region

GC content^a

PA14 gene ID

Previously assigned gene name

Gene function

Correlation coefficient of gene with rank order of virulence

Position of gene in Figure 4c

PA14R09

14

58.5

PA14_03370^b

Unknown

0.02

1

PA14R38

12

46.4

PA14_23420^b

ORF_10

O-antigen biosynthesis

2^c

PA14_23430

ORF_11

O-antigen biosynthesis

3^c

PA14R41

8

66

PA14_27680^b

C118

Unknown

0.63

4

PA14_27700^b

C120

Putative transcriptional regulator

0.63

5

PA14R77

3

53.9

PA14_58760

pilC

Type 4 fimbrial biogenesis

Not on oligo array^c

PA14R78

112

59.7

PA14_59010^b

RL107

Unknown

0.44

6

PA14_59070

RL102

Unknown

0.44

7

PA14R79

2

54.9

PA14_60290^b

pilW

Type 4 fimbrial biogenesis

Not on oligo array^c

^aGC content of the PA14 genome is 66.3 ± 4.3%. ^bThese mutants were grown on minimal media and all had wild-type growth curves. ^cThese gene clusters are present in all P. aeruginosa isolates, but the sequences are highly divergent. Oligos for the PA14-specific O-antigen cluster were included on the array, but none of the other strains contained the PA14-version of these genes. The two clusters involved in type 4 fimbrial biogenesis were not included on the array because the sequences were not sufficiently different to pass our criteria for oligo design (see Materials and methods).

Lee et al. Genome Biology 2006 7:R90   doi:10.1186/gb-2006-7-10-r90