Table 2

P[GT1]-element insertion lines with transposon insertions at genes with ethanol-dependent altered transcriptional regulation

Gene symbol

P-element insertion site

Biological process

Canton S* background

Mean elution time (min)^† (1st exposure)

Time shift (between 1st and 2nd exposures)

Slow down-regulation

Fkbp13

In 1st intron

Protein folding

A

6.7 ± 0.25

2 min^‡ ↑

Sir2

410 bp in exon 1

Regulation of transcription, DNA-dependent; chromatin silencing

B

5.0 ± 0.2

2 min^§ ↓

Bubblegum^H

In 1st intron

Fatty acid metabolism

C

6.2 ± 0.21

0.5 min (NS) ↓

Acute up-regulation

CG1516^H

125 bp upstream of transcription initiation site of the longest transcript

Pyruvate metabolism; fatty acid biosynthesis

E

4.2 ± 0.12

1 min (NS) ↑

Transient up-regulation

Thor

431 bp upstream of transcription initiation site

Negative regulation of translational initiation; response to stress; defense response

E

7.5 ± 0.11; R

2.5 min^§ ↑

Pyruvate dehydrogenase kinase^H

47 bp in exon 1

Pyruvate metabolism; tricarboxylic acid cycle; regulation of phosphate metabolism

F

7.4 ± 0.30; R

1 min^‡ ↑

Slow up-regulation

v(2)k05816^H

281 bp upstream of transcription initiation site

Fatty acid biosynthesis

A

6.1 ± 0.25

1 min (NS) ↑

elbow B^H

888 bp upstream of transcription initiation site

Tracheal system development

A

6.8 ± 0.26

0.5 min (NS) ↓

Hormone receptor-like in 38

1,428 kb upstream of transcription initiation site

Regulation of transcription from RNA polymerase II promoter; intracellular signaling cascade

A

5.8 ± 0.22; S

1 min (NS) ↓

nuclear fallout^H

In 4th intron

Actin cytoskeleton reorganization; microtubule-based process

B

6.6 ± 0.30

1 min (NS) ↓

CG12505

136 bp upstream of transcription initiation site

Unknown

B

4.8 ± 0.18

1.5 min^‡ ↓

tramtrack

219 bp in exon 1 of the longest transcript

Regulation of transcription from RNA polymerase II promoter; peripheral nervous system development

B

5.8 ± 0.20

1 min^§ ↑

CG9086^H

50 bp in exon 1

Protein ubiquitination

B

6.1 ± 0.18

1.5 min^§ ↓

CG6767

In 1st intron

Nucleotide biosynthesis, metabolism

B

7.0 ± 0.16; R

0.5 min (NS) ↑

CG32434

36 bp upstream of transcription initiation site

Unknown

B

6.1 ± 0.26

2 min^§ ↓

frizzled 2^H

147 bp in exon 1

Signal transduction; G-protein coupled receptor protein signaling pathway

E

5.9 ± 0.13; R

2 min^§ ↑

Malic enzyme^H

In 1st intron

Malate metabolism; tricarboxylic acid cycle

E

6.4 ± 0.15; R

6 min^¶ ↑

CG9238

73 bp in exon 1

Glycogen metabolism

F

7.6 ± 0.12; R

3.5 min^§ ↑

spalt major^H

154 bp upstream of transcription initiation site

Tracheal system development; sensory organ development; negative regulation of transcription

F

6.9 ± 0.18; R

0.5 min (NS) ↓

lola

In 1st intron

Transmission of nerve impulse; regulation of transcription from RNA polymerase II promoter

F

6.1 ± 0.11; R

3 min^§ ↑

*See 5 for mean elution times of P-element free Canton S genetic backgrounds A, B, C, E and F. ^†S and R designate P-element insert lines that are significantly more sensitive or more resistant than the co-isogenic control, respectively (one-way ANOVA). An H after a gene symbol indicates genes that have human orthologues. Up and down arrows designate increase or decrease, respectively, in the time shift between the first and second ethanol exposure compared with the co-isogenic control (Student's t-test). ^‡p < 0.05, ^§p < 0.01, ^¶p < 0.001. bp, base-pairs; NS, not significant.

Morozova et al. Genome Biology 2006 7:R95   doi:10.1186/gb-2006-7-10-r95