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Open Access Research

Global analysis of mRNA stability in the archaeon Sulfolobus

Anders F Andersson12*, Magnus Lundgren3, Stefan Eriksson3, Magnus Rosenlund4, Rolf Bernander3 and Peter Nilsson1

Author Affiliations

1 Department of Gene Technology, School of Biotechnology, KTH - Royal Institute of Technology, AlbaNova University Center, SE-106 91 Stockholm, Sweden

2 Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720-4767, USA

3 Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, SE-752 36 Uppsala, Sweden

4 Department of Mathematics, KTH - Royal Institute of Technology, SE-100 44 Stockholm, Sweden

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Genome Biology 2006, 7:R99  doi:10.1186/gb-2006-7-10-r99

Published: 26 October 2006

Additional files

Additional data file 1:

A table with mRNA half-life data for 2,064 and 1,582 genes in S. solfataricus and S. acidocaldarius, respectively

Format: XLS Size: 3.1MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional data file 2:

Scatterplots of mRNA half-life for upstream (x-axis) versus downstream (y-axis) gene in gene pairs encoded on the same strand and separated by < 10 nucleotides in S. solfataricus

Format: PDF Size: 62KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional data file 3:

A scatterplot of mRNA half-lives of nine S. solfataricus genes, derived by qPCR (x-axis) and microarray (y-axis) analysis

Format: PDF Size: 19KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional data file 4:

A figure displaying the distributions of S. acidocaldarius mRNA half-lives for different functional categories of genes in the COG database

Format: PDF Size: 65KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional data file 5:

A figure showing mRNA half-life in relation to transcript abundance, putative operon length (in number of genes) and putative transcript length (in nucleotides) in S. acidocaldarius

Format: PDF Size: 133KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional data file 6:

Flow cytometry of S. solfataricus, showing cell size (left) and DNA content (right) distributions of samples collected at different time points after, and immediately before (0 minutes), actinomycin D addition

Format: BMP Size: 140KB Download file

Open Data