Global analysis of mRNA stability in the archaeon Sulfolobus

Anders F Andersson¹^,2 , Magnus Lundgren³ , Stefan Eriksson³ , Magnus Rosenlund⁴ , Rolf Bernander³ and Peter Nilsson¹

¹Department of Gene Technology, School of Biotechnology, KTH - Royal Institute of Technology, AlbaNova University Center, SE-106 91 Stockholm, Sweden

²Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720-4767, USA

³Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, SE-752 36 Uppsala, Sweden

⁴Department of Mathematics, KTH - Royal Institute of Technology, SE-100 44 Stockholm, Sweden

author email corresponding author email

Genome Biology 2006, 7:R99doi:10.1186/gb-2006-7-10-r99


Published:	26 October 2006

Subject areas: Microbiology and parasitology, Cell biology, Molecular biology

Additional files

Additional data file 1:

A table with mRNA half-life data for 2,064 and 1,582 genes in S. solfataricus and S. acidocaldarius, respectively

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Additional data file 2:

Scatterplots of mRNA half-life for upstream (x-axis) versus downstream (y-axis) gene in gene pairs encoded on the same strand and separated by < 10 nucleotides in S. solfataricus

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Additional data file 3:

A scatterplot of mRNA half-lives of nine S. solfataricus genes, derived by qPCR (x-axis) and microarray (y-axis) analysis

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Additional data file 4:

A figure displaying the distributions of S. acidocaldarius mRNA half-lives for different functional categories of genes in the COG database

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Additional data file 5:

A figure showing mRNA half-life in relation to transcript abundance, putative operon length (in number of genes) and putative transcript length (in nucleotides) in S. acidocaldarius

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Additional data file 6:

Flow cytometry of S. solfataricus, showing cell size (left) and DNA content (right) distributions of samples collected at different time points after, and immediately before (0 minutes), actinomycin D addition

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