Genome Biology

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Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors

Margarida Gama-Carvalho1*, Nuno L Barbosa-Morais1,2, Alexander S Brodsky3,4, Pamela A Silver3 and Maria Carmo-Fonseca1

Author Affiliations

1 Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal

2 Hutchison/MRC Research Centre, Department of Oncology, University of Cambridge, Hills Road, Cambridge CB2 0XZ, UK

3 Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Alpert 536, Boston, MA 02115, USA

4 Department of Molecular Biology, Cell Biology and Biochemistry and Center for Genomics & Proteomics, Brown University, 69 Brown Street, Providence, Rhode Island 02912, USA

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Genome Biology 2006, 7:R113 doi:10.1186/gb-2006-7-11-r113

Published: 30 November 2006

Additional files

Additional data file 1:

Figure presenting detailed results regarding the initial steps in the microarray data analysis.

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Additional data file 2:

Lists of mRNAs associated with U2AF65 and PTB and control nonassociated mRNA populations.

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Additional data file 3:

Methods used for sequence analysis of consensus binding motifs.

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Additional data file 4:

Lists of the subsets of mRNAs that were subject to sequence analysis of consensus binding motifs and the results of this analysis.

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Additional data file 5:

Primer sequences used.

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