Email updates

Keep up to date with the latest news and content from Genome Biology and BioMed Central.

Meeting report

Moonlight in Vermont illuminates plant development

Olivier Voinnet1* and Kim Baumann2

Author affiliations

1 Institut de Biologie Moléculaire des Plantes du CNRS, rue du Général Zimmer, 67084 Strasbourg, France

2 Mediant Cambridge, Miller's Yard, Mill Lane, Cambridge CB2 1RQ, UK

For all author emails, please log on.

Citation and License

Genome Biology 2006, 7:334  doi:10.1186/gb-2006-7-12-334

The electronic version of this article is the complete one and can be found online at: http://genomebiology.com/2006/7/12/334


Published:29 December 2006

© 2006 BioMed Central Ltd

A report on the FASEB summer research conference 'Mechanisms in plant development', Saxtons River, USA, 5-9 August 2006.

Meeting report

This year's FASEB meeting on plant development covered a wide range of topics, including patterning, cell specification and the evolution of developmental mechanisms. The plant hormone auxin and some recently discovered small regulatory RNAs emerged clearly as key regulators of many developmental processes. Also notable was the variety of tools involved in the work presented, ranging from molecular genetics and genomics to imaging and computational modeling.

A feast of auxin

Many presentations showed how highly specific patterns of auxin distribution strongly influence plant growth and development. Establishment and maintenance of such patterns depend largely on active transport mechanisms mediated by the PIN family of efflux carriers, which localize to the plasma membrane at one pole of the cell. Cellular responses to auxin involve rapid changes in gene expression regulated by auxin response factors (ARFs) and AUX/IAA proteins. ARFs bind directly to DNA to activate (or repress) transcription, a process inhibited by their heterodimerization with AUX/IAAs. Auxin derepresses ARF functions by promoting the ubiquitination and degradation of AUX/IAAs.

During embryogenesis, the zygote undergoes cell division, growth and cell-type specification events that ensure the correct positioning of embryonic organs. These early events establish the plant body plan by defining the shoot and root positions (called poles) and the upper (adaxial) and lower (abaxial) surfaces of leaves. The first Arabidopsis asymmetric zygotic division produces a small apical cell, which generates the proembryo, and a larger basal cell, which forms the extraembryonic suspensor attaching the embryo to maternal tissues. The proembryo then recruits the uppermost suspensor cell to form the root meristem founder cell (the hypophysis). PIN-dependent polar auxin transport and activity of ARF5 (also called MONOPTEROS, MP) and IAA12 (also called BODENLOS, BDL) are all required for specification of the hypophysis. However, during the time of hypophysis specification, MP and BDL are expressed only in the apical cell derivatives, suggesting that they act non-cell-autonomously to specify the hypophysis (they are required in cells other than those in which they are produced).

Dolf Weijers (Wageningen University, Wageningen, The Netherlands) showed that MP and BDL do not themselves move but, rather, they generate downstream signals from proembryonic central cells to the immediately adjacent hypophysis. One mobile signal is auxin itself, because BDL-dependent MP activity induces PIN1 expression and auxin translocation to the hypophysis. However, auxin is insufficient for hypophysis specification, a finding that prompted the search for additional target genes of MP that potentially act non-cell-autonomously. A promising candidate, TARGET OF MONOPTEROS3 (TOM3), is indeed transcribed in the upper cells of the embryo, whereas its protein product, a putative AP2-domain transcription factor, accumulates in the hypophysis. Jeff Long (Salk Institute, La Jolla, USA) provided clues to how embryonic apical fates are specified, a process compromised at the transitional stage in the tpl-1 dominant-negative allele of TOPLESS (TPL), which encodes a transcriptional co-repressor. In tpl-1, shoot poles are transformed into root poles, giving rise to double-rooted seedlings. This phenotype provided the basis for an ethyl methane sulfonate (EMS) suppressor screen, leading to the identification of big top (bgt, encoding a histone acetyltransferase) and top heavy (tph, a new allele of PHABULOSA, PHB). Thus PHB, a class III homeodomain-leucine zipper (HD-ZIP) transcription factor required for adaxialization in post-embryonic tissues, might also specify shoot pole identity during embryogenesis. A yeast two-hybrid screen for TPL-interacting proteins identified transcription factors from different families - all containing the ERF-associated amphiphilic repression (EAR) domain - together with AUX/IAA proteins. Long suggested that TPL normally co-represses ARF-mediated transcription and that, in tpl-1 mutants, repression remains unaffected by auxin because tpl-1 suppresses the bdl phenotype.

The HD-ZIPIII and KANADI (KAN) family of genes have complementary expression patterns and antagonistic activity in the specification of organ polarity (Figure 1b). John Bowman (Monash University, Melbourne, Australia) showed how both these classes of transcription factors can affect vascular differentiation and basic body-plan development through the control of auxin distribution. Ectopic organs and vascular bundles in kan mutants and lack of bilateral symmetry and shoot apical meristems (SAMs) in hd-zipIII mutants all correlated with altered PIN1 expression. Bowman proposed that the KAN genes negatively regulate auxin flow by regulating the PIN genes whereas the HD-ZIPIII genes are positively regulated by auxin. He also proposed that apical and vascular meristems share common patterning mechanisms involving HD-ZIPIII and KAN activities. The conservation of these genes in all land plants suggests that they have ancestral functions in establishing three-dimensional growth patterns, functions that preceded the evolution of leaf vascularization.

thumbnailFigure 1. A model integrating the multiple layers of regulation that account for the development and polarization of leaf primordia from the shoot apical meristem (SAM) in plants. (a) The role of auxin effluxes, based on the models of Meyerowitz and Kuhlemeier. Top view of a shoot, showing the SAM (inside the dotted line) and three developing leaf primordia at different stages. Dark green, high levels of auxin; light green, low auxin levels; arrows, movement of auxin; PIN1 and CUC2 are expressed in the high and low auxin regions, respectively. In both models, the PIN1 distribution in a given cell depends on the relative auxin concentration in neighboring cells. PIN1 preferentially polarizes towards adjacent cells with the highest auxin content, pumping more auxin into these cells. (b) Regulation of gene expression by transcription factor proteins. Expression of ASYMMETRIC LEAVES1 (AS1) is restricted to the leaf primordia; this maintains the repression of KNOX proteins in the leaf primordia and enables leaf initiation when auxin accumulates. KNOXs, which specify meristem identity, are thus confined to the SAM. HD-ZIPIII and KANADI (KAN) genes have complementary expression patterns in leaf primordia; they act antagonistically to each other to specify the polarity of the emerging leaf. (c) A close-up of the leaf primordium in (b), showing opposing gradient of protein and small RNA expression. Cell-to-cell movement might cause a reduction in levels of TAS3 from the SAM vasculature to the abaxial side of the developing primordium. This might confine HD-ZIPIII expression to the adaxial domain. The model is based on the proposal by Timmermans that miR166 expression is transcriptionally controlled by ETT, which is itself a known target of TAS3. (d) The pathway of ta-siRNA biogenesis. First, a non-coding transcript is cleaved by the miRNA-loaded Argonaute1 (AGO1), the 'slicer' component of the RISC complex. Cleavage fragments are then converted into dsRNA by SGS3 and RDR6, from which ta-siRNAs are generated by the action of DCL4. The ta-siRNAs then cleave and degrade target transcripts bearing sequences complementary to the ta-siRNA, possibly through the recruitment of an AGO7-programmed RISC.

Many talks showed the importance of auxin in organogenesis and in specifying the regular arrangement of lateral organs around the stem (phyllotaxis). This process depends on the SAM, a group of undifferentiated cells producing organ primordia and new meristems at its flanks. Cris Kuhlemeier (Berne University, Berne, Switzerland), who presented work done in collaboration with Przemyslaw Pruswinkiewicz (Calgary University, Calgary, Canada) and Elliot Meyerowitz (Cal-Tech, Pasadena, USA), illustrated the benefits of mathematical modeling for understanding the role of auxin in patterning. The speakers presented related models that assume that patterning occurs in the SAM epidermal layer, where auxin polarizes its own efflux towards newly emerging primordia through the action of PIN1. In both models, PIN1 distribution in a given cell depends on the relative auxin concentration in neighboring cells. PIN1 preferentially polarizes towards adjacent cells with the highest auxin content, pumping even more auxin into these cells, thereby creating regular alternation of auxin-enriched and auxin-depleted areas (Figure 1a). When integrated into models of normally growing meristems, Arabidopsis phyllotaxis was convincingly reproduced. Meyerowitz's model could also predict rapid PIN1 polarization reversals during primordium development.

Despite these advances, important questions remain unapproachable by predictive models, for example, how cells sense auxin concentrations, how auxin influences PIN polarity and how it affects growth and patterning. Michael Sauer (Tübingen University, Tübingen, Germany) provided evidence that AUX/IAA pathways control the tissue-specific reorientation of PINs, and Meyerowitz showed how three-dimensional time-lapse imaging of inflorescence meristems could help understanding of growth and patterning. Cell divisions were monitored over time at the same time as the dynamic expression of several transcription factors was measured by labeling them with green fluorescent protein (GFP): the factors were ones required for meristem maintenance (SHOOT MERISTEMLESS, STM) and organ separation (CUP-SHAPED COTYLEDON2, CUC2) or for organ polarity (REVOLUTA, REV; FILAMENTOUS FLOWER). Combined with analyses of PIN1-GFP localization (which reports cell division and auxin distribution), the results suggested that auxin transport, besides determining the position of organ primordia, also regulates their subsequent differentiation by controlling expression of these genes. Interestingly, primordia can emerge where expression of adaxial fate-specifying factors (such as REV, which is auxin-dependent) and abaxial fate-specifying factors (such as KAN, which is auxin-independent) coincide, reminiscent of the overlap between abaxial and adaxial fates required for leaf blade expansion.

In plants with simple leaves (such as Arabidopsis), expression of KNOX transcription factors (including BREVIPEDI-CELLUS, BP) is confined to the meristem by the activity of actin-related proteins (ARPs) such as ASYMMETRIC LEAVES1 (AS1; Figure 1b). In Arabidopsis, restricted expression of AS1 in leaf primordia maintains the repression of BP and enables leaf initiation, coincident with auxin accumulation. Angela Hay (Oxford University, Oxford, UK) proposed that the AS1 and auxin pathways converge to negatively regulate BP, because the as1 mutant phenotype is enhanced in an auxin-insensitive background (auxin-resistant1): leaves had dramatically lobed margins and ectopic stipules as a result of ectopic BP expression. Hay also discussed how disrupted auxin gradients, resulting from BP misexpression, can alter leaf shape.

Miltos Tsiantis (Oxford University) showed that, in contrast to the exclusion of KNOXs from Arabidopsis leaves, their expression is required to delay differentiation and produce dissected leaves in the close relative Cardamine hirsuta. Differences in KNOX expression between Arabidopsis and C. hirsuta arose through changes in their regulatory sequences, even though AS1 expression and function is conserved between the two species, indicating that evolutionary tinkering with KNOX regulation, constrained by ARP function, has produced diverse leaf forms. The existence of a developmental constant in the shoot-leaf transition involving KNOX/ARP proteins was evidenced by Jane Langdale (Oxford University, UK), who showed these factors were recruited at least twice independently in land plant evolution (in lycophytes and euphyllophytes) to generate different types of leaves. Finally, Neelima Sinha (University of California, Davis, USA) used global gene expression profiling data and quantitative trait locus analyses to isolate additional factors regulating leaf complexity in Solanum species, also revealing a role for the polarity gene PHANTASTICA in such a process.

Small regulatory RNAs

Small interfering RNAs (siRNAs) and microRNAs (miRNAs) have emerged as essential regulators of eukaryotic gene expression. In particular, miRNAs have been shown to be crucial in plant and animal development. In plants, the RNAse III enzyme Dicer-like-1 (DCL1) processes miRNAs from imperfect stem-loop RNAs transcribed from intergenic or intronic regions. Upon incorporation into an RNA-induced silencing complex (RISC), plant miRNAs are believed to cleave cellular transcripts bearing miRNA-complementary sequences, but the underlying mechanism remains poorly understood.

One of us (O.V.) presented the results of a forward-genetic screen using a GFP-based sensor mRNA with a fully complementary miRNA-binding site at its 3' end and suggested that, in addition to specifying mRNA cleavage, plant miRNAs might also promote widespread translational inhibition and/or protein degradation. He also suggested that the second process specifically inactivated in certain mutants uncovered by this screen might occur in dedicated sub-cellular structures.

Leslie Sieburth (University of Utah, Salt Lake City, USA) presented a screen for Arabidopsis mutants with cotyledon and leaf vein patterning defects that led to identification of plant orthologs of two major constituents of P-Bodies, in which mRNA decay and miRNA-directed translational inhibition are known to occur in animals. VARICOSE (VCS) is homologous to a WD-domain protein that facilitates interactions between the human mRNA decapping enzymes hDCP2 and hDCP1; TRIDENT (TDT) is orthologous to hDCP2 itself. Sieburth showed that cellular target transcripts of all (or several) miRNAs accumulate normally in tdt and vcs mutants, although the miRNA target protein levels were not tested. TDT:GFP fusions form cytoplasmic speckles reminiscent of P-bodies.

In Arabidopsis leaves, abaxial expression of the miRNAs miR165 and miR166 directs degradation of the PHB and PHAVOLUTA (PHV) mRNAs, thereby restricting their accumulation to adaxial domains (Figure 1c). Marja Timmermans (Cold Spring Harbor Laboratory, New York, USA) showed that, similarly, miR166 targets the HD-ZIPIII family member rolled leaf1 (rld1) to specify adaxia-abaxial polarity in maize leaves. Levels of miR166 peak below incipient leaves and form a gradient, suggesting that miR166 is spatiotemporally regulated by a mobile signal, or might itself be mobile (Figure 1c). Timmermans reported that leafbladeless1 (lbl1) is required for proper rdl1 and miR166 expression. The lbl1 gene is orthologous to Arabidopsis SUPPRESSOR OF GENE SILENCING3 (SGS3), required for synthesis of trans-acting (ta)-siRNAs, a newly discovered class of small RNAs. Biogenesis of ta-siRNAs involves miRNA-directed processing of primary transcripts, conversion of the resulting precursor RNAs to double-stranded RNAs through RNA-DEPENDENT RNA POLYMERASE6 (RDR6) and SGS3 activities, and phased DICER-LIKE4 (DCL4)-mediated processing (Figure 1d).

Scott Poethig (University of Pennsylvania, Philadelphia, USA) reported that RDR6 and SGS3 were originally identified through a genetic screen for Arabidopsis mutants with a compromised juvenile-adult phase transition. Transcript profiling and searches for suppressors of rdr6 and sgs3 converged in the identification of ETTIN (ETT, also called ARF3) and ARF4. Both transcription factors are targeted by TAS3, an evolutionarily conserved ta-siRNA whose accumulation requires miR390-directed cleavage (Figure 1d). Expression patterns and loss-of-function analyses suggest that ETT and ARF4 regulate the sensitivity of juvenile-adult phase changes to a temporal, possibly mobile signal. Interestingly, Timmermans showed that loss of lbl1 function reduces accumulation of ta-siR2142, the maize TAS3 ortholog. Binding sites for ARF3 and ARF4 (both targeted by ta-siR2142) are found in the promoters of some of the several miR166 genes in maize, and this could explain how reduced ta-siR2142 levels cause misexpression of miR166 in lbl1 mutant leaves. Thus, miR166 transcription might normally be inhibited in adaxial domains through ta-siR2142-mediated repression of ETT and ARF4 (Figure 1c). Because ta-siR2142 accumulation is itself dependent upon miR390, this example illustrates the complexity expected from small RNA-directed developmental mechanisms.

The ta-siRNA pathway thus regulates phase change and leaf polarity by controlling ARF4 and ETT. Given that miRNAs target other ARFs and that ta-siRNA biogenesis requires miRNA-directed cleavage, why do miRNAs not directly regulate ETT and ARF4? The answer possibly lies in the fact that both Poethig's and Timmermans' models involve mobile signals. However, miRNAs act in a spatially restricted manner. By contrast, siRNAs exert their effects away from their sites of synthesis and amplification by DCL4 and RDR6, respectively. As endogenous RDR6 and DCL4 products, ta-siRNAs thus have all the required features to signal adaxial fates between cells (Figure 1c) and perhaps phase changes in whole organisms. It is in fact conceivable that ta-siRNA pathways could have evolved specifically to convey the regulatory effects of cell-autonomous miRNAs in distant tissues.

It is becoming ever clearer that multiple layers of regulation control patterning and organ growth in plants. 'Classical' protein-based developmental programs must now be integrated into the broader contexts of hormone signaling and small RNA-directed functions. One important challenge will be to address how those networks interact with each other and how redundant they are.