Figure 2.

Target-selected mutagenesis of Oryzias latipes p53 gene. Genomic organization and protein structure of the medaka p53 gene. The region analyzed by PCR and dideoxy resequencing is indicated by bidirectional arrows. The ENU mutations are shown by solid arrows. Basic, basic regulatory region; DBD, DNA-binding domain; NLS, nuclear localization signal; Pro-rich, proline-rich domain; TAD, transactivation domain; TET, tetramerization domain.