Table 2

Medaka mutant library* screening statistics

Gene

Exons

Exons screened

Amplicons

Base-pairs screened

Exonic

Intronic

Total

Mutation rate


Stop

Missense

Silent

Intron

Splice


Blm

23

2

2

3,129,006

1

4

0

1

0

6

1/521,501

p53

11

3

1

1,854,603

2

3

0

1

1

7

1/264,943

Sirt1

9

5

2

5,767,496

0

12

0

2

1

15

1/384,500

Dcr-1

27

7

4

7,879,290

0

16

4

7

0

27

1/291,826

Parkin

11

3

1

3,461,661

1

3

3

2

0

9

1/384,629

Total

81

20

10

22,092,056

4

38

7

13

2

64

1/345,188


*The mutant library consists of 5,771 cryopreserved male progeny from ENU-mutagenized fish. Due to the compact medaka genome architecture, multiple exons can often be amplified and sequenced from a single amplicon. Determined by counting all bases in the resequencing reads that were read with phred quality >20.

Taniguchi et al. Genome Biology 2006 7:R116   doi:10.1186/gb-2006-7-12-r116

Open Data