Table 3

Detailed overview of the induced mutations retrieved from the mutant medaka library

Number

Exon

Sequence context

Amino acid change

Type of mutation


Dicer (Dcr-1)

1

10_11

5'-GATCCTTAGG (A>G) ACAAATGCTC-3'

N578D

Substitution

2

10_11

5'-GTGGTTGACG (A>G) TGACAACATC-3'

D597G

Substitution

3

10_11

5'-ACCGTCAACA (C>A) AGCCATCGGT-3'

T619K

Substitution

4

10_11

5'-CGTCAACACA (G>A) CCATCGGTCA-3'

A620T

Silent

5

10_11

5'-CAGGTACCTG (C>T) CCTGCTTTGA-3'

Intron

6

10_11

5'-CCTGCCCTGC (T>A) TTGATGTGGA-3'

Intron

7

10_11

5'-AGAATTAACT (T>A) CAACTCAACA-3'

Intron

8

16_17

5'-ATTTTTGACT (T>A) GAATAGTTGG-3'

Intron

9

16_17

5'-GAGGCTCGCA (C>T) TGGCATTCCT-3'

T897I

Substitution

10

16_17

5'-CGCACTGGCA (T>G) TCCTACCACT-3'

I899S

Substitution

11

16_17

5'-ACTACCAGGA (C>A) GCTGTCATCA-3'

D919E

Substitution

12

16_17

5'-GCTCCTTCAG (T>A) GAAACTCTTG-3'

Intron

13

16_17

5'-TCTCCATAGA (T>A) ATCGTAACTT-3'

Y926N

Substitution

14

16_17

5'-CCATAGATAT (C>T) GTAACTTTGA-3'

R927C

Substitution

15

16_17

5'-GCCACTCAGC (A>G) AGTTTCCTTC-3'

K949E

Substitution

16

16_17

5'-TTCCTTCACC (A>T) GAATACGAGA-3'

P953P

Silent

17

16_17

5'-ACCTGTCAAA (T>A) CTGAACCAGC-3'

N972K

Substitution

18

20a

5'-GGTTTTTGTG (T>C) CAGATATCCA-3'

Intron

19

20a

5'-CCATTGACAA (C>A) AAAGCTTACA-3'

N1094K

Substitution

20

20a

5'-AAGCTTACAG (T>A) TCTTGCTCCG-3'

S1098R

Substitution

21

20a

5'-TTGCTCCGAG (T>C) CCTGCAGCGA-3'

S1103P

Substitution

22

20a

5'-GCTCAGAACC (T>G) GCCCTCTCAG-3'

P1120P

Silent

23

20a

5'-CCTTCACCAA (C>T) CTGACAGCTG-3'

P1168S

Substitution

24

22b

5'-AATAAGGCCT (A>G) CCTGCTGCAA-3'

Y1635C

Substitution

25

25_26

5'-AGGAAGAGGA (C>T) ATTGAGGTCC-3'

D1754D

Silent

26

25_26

5'-TTCATCACTG (T>A) TGTTGGAGAT-3'

Intron

27

25_26

5'-CTGCTGGAGA (T>A) GGAGCCGGAA-3'

M1813K

Substitution

p53

1

5_6_7

5'-TCCCTTTTCT (C>T) CATCGACTGT-3'

Intron

2

5_6_7

5'-TGGCCCAGTA (T>A) TTTGAAGACC-3'

Y186X

Truncation

3

5_6_7

5'-CTACATGTGT (A>G) ACAGCTCGTG-3'

N220D

Substitution

4

5_6_7

5'-TACATGTGTA (A>G) CAGCTCGTGC-3'

N220S

Substitution

5

5_6_7

5'-GTGTAACAGC (T>C) CGTGCATGGG-3'

S222P

Substitution

6

5_6_7

5'-TCTGGAAACC (G>T) AGTAAGTTTA-3'

E241X

Truncation

7

5_6_7

5'-GGAAACCGAG(T>C)AAGTTTAGTC-3'

Splice

Sirt1

1

2_3_4

5'-CGATGACGGA (T>A) CCTCTCATGC-3'

S138T

Substitution

2

2_3_4

5'-CTAGTTCCAG (C>G) GACTGGACTC-3'

S144R

Substitution

3

2_3_4

5'-AGTTCCAGCG (A>G) CTGGACTCCG-3'

D145G

Substitution

4

2_3_4

5'-AGCGACTGGA (C>T) TCCGCAGCCC-3'

T147I

Substitution

5

2_3_4

5'-CAGCCCCAGA (T>A) CGGTCAGAAT-3'

I152N

Substitution

6

2_3_4

5'-AAGCCGTTGT (G>T) AGCTCAGGTG-3'

Intron

7

2_3_4

5'-CCCGAGACCA (T>C) ACTCCCACCC-3'

I179T

Substitution

8

2_3_4

5'-CTGTGGCAGA (T>C) CATCATCAAC-3'

I192T

Substitution

9

2_3_4

5'-ATCATGGTTC (T>C) GACCGGTGCA-3'

L227P

Substitution

10

2_3_4

5'-CGGTGCAGGT (G>T) TAGGTGTTTC-3'

Splice

11

2_3_4

5'-TAAAGAAACG (G>A) TAAACACCGG-3'

Intron

12

2_3_4

5'-CGGCTTGCTG (T>C) CGACTTTCCC-3'

V253A

Substitution

13

5_6

5'-AACATCGACA (C>A) GCTGGAACAA-3'

T317K

Substitution

14

5_6

5'-TGCGACGGCT (T>C) CCTGTCTCGT-3'

S338P

Substitution

15

5_6

5'-CGTTTGTAAA (C>A) ACAAAGTGGA-3'

H344N

Substitution

Blm

1

5_6

5'-AGCAGTAGGG (C>T) AATCTGTGTG-3'

A477V

Substitution

2

5_6

5'-TGTGACTCTC (T>G) ATCAACTCCC-3'

L489R

Substitution

3

5_6

5'-ACTTCTAAAA (C>T) AACCTTGTTT-3'

Q497X

Truncation

4

5_6

5'-TTTCTCAGAG (A>G) GCACAAGTCG-3'

S503G

Substitution

5

7

5'-TATTTTCTAT (C>T) TTCATTCAGA-3'

Intron

6

7

5'-CTTGATGCCC (A>G) CAGGTTGGTG-3'

T670A

Substitution

Parkin (Park2)

1

9_10_11

5'-ATGCACGGTA (C>G) CAGCAATATG-3'

Y314X

Truncation

2

9_10_11

5'-GACTCATGTG (T>C) CCGGCACCTG-3'

C331C

Silent

3

9_10_11

5'-AGGGTGGAGT (G>T) TGAGAGACAG-3'

C351F

Substitution

4

9_10_11

5'-GCTGTGGCTT (T>A) GTCTTCTGTA-3'

F359L

Substitution

5

9_10_11

5'-TTTTGTGATG (A>T) CATTGCCGTG-3'

Intron

6

9_10_11

5'-GTCTTATTCA (G>A) GAGATGACCA-3'

Q410Q

Silent

7

9_10_11

5'-TCTCCACCTG (C>T) AGGTGGCTGC-3'

Intron

8

9_10_11

5'-TGCACATGCA (T>C) TGTGCTCTGT-3'

H433H

Silent

9

9_10_11

5'-AGGGAGTGCA (T>A) GGGAAACCAC-3'

M454K

Substitution


Taniguchi et al. Genome Biology 2006 7:R116   doi:10.1186/gb-2006-7-12-r116

Open Data