Table 3 |
||||
|
Detailed overview of the induced mutations retrieved from the mutant medaka library |
||||
|
Number |
Exon |
Sequence context |
Amino acid change |
Type of mutation |
|
|
||||
|
Dicer (Dcr-1) |
||||
|
1 |
10_11 |
5'-GATCCTTAGG (A>G) ACAAATGCTC-3' |
N578D |
Substitution |
|
2 |
10_11 |
5'-GTGGTTGACG (A>G) TGACAACATC-3' |
D597G |
Substitution |
|
3 |
10_11 |
5'-ACCGTCAACA (C>A) AGCCATCGGT-3' |
T619K |
Substitution |
|
4 |
10_11 |
5'-CGTCAACACA (G>A) CCATCGGTCA-3' |
A620T |
Silent |
|
5 |
10_11 |
5'-CAGGTACCTG (C>T) CCTGCTTTGA-3' |
Intron |
|
|
6 |
10_11 |
5'-CCTGCCCTGC (T>A) TTGATGTGGA-3' |
Intron |
|
|
7 |
10_11 |
5'-AGAATTAACT (T>A) CAACTCAACA-3' |
Intron |
|
|
8 |
16_17 |
5'-ATTTTTGACT (T>A) GAATAGTTGG-3' |
Intron |
|
|
9 |
16_17 |
5'-GAGGCTCGCA (C>T) TGGCATTCCT-3' |
T897I |
Substitution |
|
10 |
16_17 |
5'-CGCACTGGCA (T>G) TCCTACCACT-3' |
I899S |
Substitution |
|
11 |
16_17 |
5'-ACTACCAGGA (C>A) GCTGTCATCA-3' |
D919E |
Substitution |
|
12 |
16_17 |
5'-GCTCCTTCAG (T>A) GAAACTCTTG-3' |
Intron |
|
|
13 |
16_17 |
5'-TCTCCATAGA (T>A) ATCGTAACTT-3' |
Y926N |
Substitution |
|
14 |
16_17 |
5'-CCATAGATAT (C>T) GTAACTTTGA-3' |
R927C |
Substitution |
|
15 |
16_17 |
5'-GCCACTCAGC (A>G) AGTTTCCTTC-3' |
K949E |
Substitution |
|
16 |
16_17 |
5'-TTCCTTCACC (A>T) GAATACGAGA-3' |
P953P |
Silent |
|
17 |
16_17 |
5'-ACCTGTCAAA (T>A) CTGAACCAGC-3' |
N972K |
Substitution |
|
18 |
20a |
5'-GGTTTTTGTG (T>C) CAGATATCCA-3' |
Intron |
|
|
19 |
20a |
5'-CCATTGACAA (C>A) AAAGCTTACA-3' |
N1094K |
Substitution |
|
20 |
20a |
5'-AAGCTTACAG (T>A) TCTTGCTCCG-3' |
S1098R |
Substitution |
|
21 |
20a |
5'-TTGCTCCGAG (T>C) CCTGCAGCGA-3' |
S1103P |
Substitution |
|
22 |
20a |
5'-GCTCAGAACC (T>G) GCCCTCTCAG-3' |
P1120P |
Silent |
|
23 |
20a |
5'-CCTTCACCAA (C>T) CTGACAGCTG-3' |
P1168S |
Substitution |
|
24 |
22b |
5'-AATAAGGCCT (A>G) CCTGCTGCAA-3' |
Y1635C |
Substitution |
|
25 |
25_26 |
5'-AGGAAGAGGA (C>T) ATTGAGGTCC-3' |
D1754D |
Silent |
|
26 |
25_26 |
5'-TTCATCACTG (T>A) TGTTGGAGAT-3' |
Intron |
|
|
27 |
25_26 |
5'-CTGCTGGAGA (T>A) GGAGCCGGAA-3' |
M1813K |
Substitution |
|
p53 |
||||
|
1 |
5_6_7 |
5'-TCCCTTTTCT (C>T) CATCGACTGT-3' |
Intron |
|
|
2 |
5_6_7 |
5'-TGGCCCAGTA (T>A) TTTGAAGACC-3' |
Y186X |
Truncation |
|
3 |
5_6_7 |
5'-CTACATGTGT (A>G) ACAGCTCGTG-3' |
N220D |
Substitution |
|
4 |
5_6_7 |
5'-TACATGTGTA (A>G) CAGCTCGTGC-3' |
N220S |
Substitution |
|
5 |
5_6_7 |
5'-GTGTAACAGC (T>C) CGTGCATGGG-3' |
S222P |
Substitution |
|
6 |
5_6_7 |
5'-TCTGGAAACC (G>T) AGTAAGTTTA-3' |
E241X |
Truncation |
|
7 |
5_6_7 |
5'-GGAAACCGAG(T>C)AAGTTTAGTC-3' |
Splice |
|
|
Sirt1 |
||||
|
1 |
2_3_4 |
5'-CGATGACGGA (T>A) CCTCTCATGC-3' |
S138T |
Substitution |
|
2 |
2_3_4 |
5'-CTAGTTCCAG (C>G) GACTGGACTC-3' |
S144R |
Substitution |
|
3 |
2_3_4 |
5'-AGTTCCAGCG (A>G) CTGGACTCCG-3' |
D145G |
Substitution |
|
4 |
2_3_4 |
5'-AGCGACTGGA (C>T) TCCGCAGCCC-3' |
T147I |
Substitution |
|
5 |
2_3_4 |
5'-CAGCCCCAGA (T>A) CGGTCAGAAT-3' |
I152N |
Substitution |
|
6 |
2_3_4 |
5'-AAGCCGTTGT (G>T) AGCTCAGGTG-3' |
Intron |
|
|
7 |
2_3_4 |
5'-CCCGAGACCA (T>C) ACTCCCACCC-3' |
I179T |
Substitution |
|
8 |
2_3_4 |
5'-CTGTGGCAGA (T>C) CATCATCAAC-3' |
I192T |
Substitution |
|
9 |
2_3_4 |
5'-ATCATGGTTC (T>C) GACCGGTGCA-3' |
L227P |
Substitution |
|
10 |
2_3_4 |
5'-CGGTGCAGGT (G>T) TAGGTGTTTC-3' |
Splice |
|
|
11 |
2_3_4 |
5'-TAAAGAAACG (G>A) TAAACACCGG-3' |
Intron |
|
|
12 |
2_3_4 |
5'-CGGCTTGCTG (T>C) CGACTTTCCC-3' |
V253A |
Substitution |
|
13 |
5_6 |
5'-AACATCGACA (C>A) GCTGGAACAA-3' |
T317K |
Substitution |
|
14 |
5_6 |
5'-TGCGACGGCT (T>C) CCTGTCTCGT-3' |
S338P |
Substitution |
|
15 |
5_6 |
5'-CGTTTGTAAA (C>A) ACAAAGTGGA-3' |
H344N |
Substitution |
|
Blm |
||||
|
1 |
5_6 |
5'-AGCAGTAGGG (C>T) AATCTGTGTG-3' |
A477V |
Substitution |
|
2 |
5_6 |
5'-TGTGACTCTC (T>G) ATCAACTCCC-3' |
L489R |
Substitution |
|
3 |
5_6 |
5'-ACTTCTAAAA (C>T) AACCTTGTTT-3' |
Q497X |
Truncation |
|
4 |
5_6 |
5'-TTTCTCAGAG (A>G) GCACAAGTCG-3' |
S503G |
Substitution |
|
5 |
7 |
5'-TATTTTCTAT (C>T) TTCATTCAGA-3' |
Intron |
|
|
6 |
7 |
5'-CTTGATGCCC (A>G) CAGGTTGGTG-3' |
T670A |
Substitution |
|
Parkin (Park2) |
||||
|
1 |
9_10_11 |
5'-ATGCACGGTA (C>G) CAGCAATATG-3' |
Y314X |
Truncation |
|
2 |
9_10_11 |
5'-GACTCATGTG (T>C) CCGGCACCTG-3' |
C331C |
Silent |
|
3 |
9_10_11 |
5'-AGGGTGGAGT (G>T) TGAGAGACAG-3' |
C351F |
Substitution |
|
4 |
9_10_11 |
5'-GCTGTGGCTT (T>A) GTCTTCTGTA-3' |
F359L |
Substitution |
|
5 |
9_10_11 |
5'-TTTTGTGATG (A>T) CATTGCCGTG-3' |
Intron |
|
|
6 |
9_10_11 |
5'-GTCTTATTCA (G>A) GAGATGACCA-3' |
Q410Q |
Silent |
|
7 |
9_10_11 |
5'-TCTCCACCTG (C>T) AGGTGGCTGC-3' |
Intron |
|
|
8 |
9_10_11 |
5'-TGCACATGCA (T>C) TGTGCTCTGT-3' |
H433H |
Silent |
|
9 |
9_10_11 |
5'-AGGGAGTGCA (T>A) GGGAAACCAC-3' |
M454K |
Substitution |
|
|
||||
|
Taniguchi et al. Genome Biology 2006 7:R116 doi:10.1186/gb-2006-7-12-r116 |
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