(a) Schematic outline of the cell culture serial dilution performed to validate our analytical model. A plate of SW620 cell culture was divided into 10 samples, each containing approximately 1 × 106 cells. One of these samples was selected at random and divided into a further 10 samples. The cell culture dilution scheme continues until 10 samples of 1 × 103 cells are achieved; there were a total number of 37 cell culture samples in our experiment. (b) Schematic outline of the RNA serial dilution that was used to control and estimate the error in our experimental data. RNA was first extracted from a plate of SW620 cell culture, then divided into 10 identical samples. One of these samples was selected at random to be further divided into 10 samples. A set of 37 controls corresponding to the cellular dilutions was obtained and used to estimate systematic variation in this analysis.
Mar et al. Genome Biology 2006 7:R119 doi:10.1186/gb-2006-7-12-r119