Figure 3.

(a) Variances calculated from the experimental data for each step of the serial dilution series; variances from the RNA dilution are represented by solid blue circles, variances from the cell culture dilution are represented by the open orange circles. (b) Estimates of biological variability obtained from the validation experiment using quant values are shown by red dots; the trend predicted by our analytical model is shown by the bold black line. Data are displayed for nine genes targeted in our validation experiment.