Table 1

Summary of splicing microarray data: unstimulated macrophages versus macrophages subjected to seven hours of LPS stimulation

Expressed unstimulated macrophages

Expressed macrophages + 7 hr LPS

Variants repressed by LPS/Tlr4 (t test P < 0.05)

Variants induced by LPS/Tlr4 (t test, P < 0.05)

Transcript framework

107

106

13

26

Total number of probes

1,404

1,376

39

88

Receptors

16 TK

Csf1r, Ifnar1

Tlr3, Cd86, Clesf9

Adapters

13 TK

Tollip

Rac2

Signal transducers

30 TK

Irak4, Map3k1, Map3k7, Mapk14, Pik3cg, Serpinb3b

Pde4a, Pik3cd, Tbk1

Transcription factors

29 TK

Atf2, Dnmt1, Sumo

Nfkbia, Nfkb1, Nfkb2, Ikbke, Irf1, Stat1, Stat2, Stat3, Sin3a

Inflammatory effectors

17 TK

Serpinb3b

Ccl3, Ccl4, Ccl5, Cxcl10, Cxcl9, Il12b, Il1b, Il6, Casp8, Tnf-α

The splicing array consisted of 1,717 oligonucleotide probes representing alternate transcripts arising from 108 TK. The experiment was repeated four times, with dye-swap. LPS, lipopolysaccharide; TK, transcriptional framework; Tlr, Toll-like receptor.

Wells et al. Genome Biology 2006 7:R10   doi:10.1186/gb-2006-7-2-r10