Identification of reproducible, differentially expressed genes from cDNA generated by each amplification method. Volcano plots show LOD for genes across four hybridizations including dye swaps of two different cell samples (OV vs 3T3). Red spots denote differentially expressed genes selected with an empirical Bayesian method with threshold at LOD score zero. The numbers of differentially expressed genes are indicated on each graph (see also Table 2). DNA, unamplified cDNA; IVTIII, three rounds of T7-based amplification; GA, global polyadenylated PCR-based amplification technique; SMART, SMART PCR-based amplification; SM37, modified SMART amplification.
Subkhankulova and Livesey Genome Biology 2006 7:R18 doi:10.1186/gb-2006-7-3-r18