Comparison of expression data generated using each of the PCR-based methods from single-cell equivalents of total RNA and from single neural stem cells. Columns 1 and 2 represent averaged expression data for dye-swap hybridizations of SMART- and GA-amplified technical replicates. Samples of (a) 1 ng, (b) 100 pg, and (c) 10 pg of total RNA were amplified by SMART or by GA in two independent amplifications and replicates were co-hybridized on microarray slides in two dye-swap hybidizations. Total RNA was extracted from a group of 12 cells obtained from developing mouse forebrain (neocortex), mixed together, divided in four equal samples and two samples were independently amplified either by SMART or GA amplification. Technical replicates generated by the same method were co-hybridized on microarray slides in two dye-swap hybridizations. The figures are averaged data for two dye-swap experiments. (d) Expression data for single neural stem cells. CR, coefficient of repeatability.
Subkhankulova and Livesey Genome Biology 2006 7:R18 doi:10.1186/gb-2006-7-3-r18