Table 1 |
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|
Selection of 30 genes considered for further Q-PCR analysis |
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|
Symbol |
Clone ID |
M |
T |
T&M |
All tumor samples (T&M) |
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|
|
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|
Intensity |
Ratio |
Min-value |
Max P value |
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|
|
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|
1 |
ABCA2 |
3508171 |
0 |
0 |
1 |
411 |
-1.4 |
4.36E-05 |
4.93E-05 |
|
2 |
ABL1 |
345666 |
1 |
0 |
0 |
569 |
-1.5 |
1.44E-04 |
1.44E-04 |
|
3 |
CASP1 |
486506 |
0 |
1 |
0 |
714 |
1.9 |
6.30E-06 |
6.93E-06 |
|
4 |
CHEK1 |
3530606 |
1 |
0 |
1 |
1,901 |
1.6 |
1.66E-05 |
3.89E-05 |
|
5 |
CHEK2 |
3532866 |
0 |
0 |
1 |
2,624 |
1.4 |
2.72E-05 |
3.77E-05 |
|
6 |
COL4A2 |
3507072 |
0 |
0 |
1 |
5,813 |
-1.6 |
7.86E-03 |
7.86E-03 |
|
7 |
COL6A1 |
3506644 |
1 |
1 |
1 |
4,268 |
-2.6 |
4.43E-12 |
3.63E-02 |
|
8 |
COL6A2 |
3347413 |
1 |
0 |
1 |
3,906 |
-4.3 |
1.37E-11 |
1.31E-02 |
|
9 |
FOS |
3688670 |
1 |
0 |
1 |
8,312 |
-1.7 |
7.39E-05 |
1.28E-04 |
|
10 |
HRASLS3 |
3051149 |
0 |
1 |
0 |
1,814 |
-1.7 |
6.22E-05 |
6.22E-05 |
|
11 |
LAMB1 |
428443 |
1 |
0 |
1 |
2,747 |
-1.5 |
5.06E-06 |
2.93E-04 |
|
12 |
MAD2L1 |
2964388 |
1 |
0 |
1 |
4,141 |
1.6 |
2.94E-05 |
1.99E-04 |
|
12 |
MMP11 |
725180 |
1 |
0 |
1 |
2,917 |
-2.3 |
1.78E-05 |
3.42E-02 |
|
14 |
PEA15 |
3346270 |
0 |
0 |
1 |
1,366 |
-1.3 |
3.48E-04 |
3.48E-04 |
|
15 |
RAD51 |
3139011 |
1 |
0 |
0 |
1,630 |
1.9 |
9.78E-03 |
9.78E-03 |
|
16 |
RECQL4 |
3634266 |
1 |
0 |
1 |
3,372 |
1.6 |
9.13E-06 |
1.76E-05 |
|
17 |
RERG |
3357341 |
1 |
1 |
1 |
294 |
-3.6 |
1.17E-05 |
3.63E-02 |
|
18 |
RRAGD |
3536088 |
1 |
0 |
1 |
596 |
-2.6 |
6.70E-07 |
4.35E-02 |
|
19 |
SERPINF1 |
2961120 |
1 |
0 |
1 |
1,902 |
-1.5 |
8.33E-10 |
2.74E-05 |
|
20 |
TGFBI |
2958878 |
1 |
1 |
1 |
13,900 |
-2.4 |
5.43E-10 |
1.31E-02 |
|
21 |
TOP2B |
470615 |
1 |
0 |
1 |
495 |
1.5 |
6.15E-06 |
2.34E-04 |
|
22 |
TP53 |
3544714 |
1 |
0 |
1 |
4,178 |
1.8 |
1.06E-06 |
1.32E-05 |
|
23 |
BCL2 |
232714 |
0 |
0 |
0 |
787 |
Not significant |
||
|
24 |
CES2 |
4298532 |
0 |
0 |
0 |
6,538 |
Not significant |
||
|
25 |
E2F4 |
3504728 |
0 |
0 |
0 |
5,620 |
Not significant |
||
|
26 |
ITGB6 |
759142 |
0 |
0 |
0 |
648 |
Not significant |
||
|
27 |
JUN |
3606344 |
0 |
0 |
0 |
2,037 |
Not significant |
||
|
28 |
RAD52 |
3614681 |
0 |
0 |
0 |
322 |
Not significant |
||
|
29 |
TGFB1 |
3356605 |
0 |
0 |
0 |
2,188 |
Not significant |
||
|
30 |
TOP1 |
502960 |
0 |
0 |
0 |
3,484 |
Not significant |
||
|
|
|||||||||
|
For each gene, symbol and Clone ID are indicated. M, T and T&M columns (1 = present, 0 = absent) specify, in the respective group of samples, which genes were statistically differentially expressed using microarrays (α = 0.01); italics refers to genes for which differential expression was 'not significant'. The relative expression levels recorded with microarrays are indicated as the ratio between resistant and sensitive samples, specified as negative (down-regulated) or positive (up-regulated) values, together with the intensity measured in resistant colon tumors. Adjusted p values were computed using z statistics with false discovery rate corrections, α = 0.01; columns 'Min p value' and 'Max p value' refer to lower and upper bound p values, respectively. |
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|
Graudens et al. Genome Biology 2006 7:R19 doi:10.1186/gb-2006-7-3-r19 |
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